Spectrum of Lactobacillus species present in healthy vagina of Indian women.

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dc.contributor.authorGarg, Kavita Bansal
dc.contributor.authorGanguli, Indrani
dc.contributor.authorDas, Ram
dc.contributor.authorTalwar, G P
dc.date.accessioned2011-12-14T03:15:39Z
dc.date.available2011-12-14T03:15:39Z
dc.date.issued2009-06
dc.description.abstractBackground & objectives: Lactobacilli are depleted in vagina of women suffering from recurring episodes of bacterial vaginosis with vaginal pH >5. With the objective of making available probiotic lactobacilli for replenishment in such women, a study was undertaken to isolate and characterize the Lactobacilli present in women with eco-healthy vagina in Delhi. No information is so far available on the species of Lactobacilli resident in vagina of women in India. Methods: Vaginal swabs were taken from 80 women with informed consent after ethical approval and grown in MRS broth. Gram-positive, catalase-negative bacilli generating about 200 bp amplicon by PCR with Lactobacillus genus specifi c primers were further characterized by employing species specifi c primers followed by sequencing of 16S rDNA. Isolates of the same species were differentiated by random amplifi ed polymorphic DNA (RAPD) profi les. Results: The predominant species isolated were L. reuteri present in 26 (32.5%) women, L. fermentum in 20 (25%), and L. salivarius in 13 (16.25%) women. Sequencing of 16S rDNA of 20 isolates showed that except for two isolates of L. plantarum, sequences of the remaining agreed well with PCR identifi cation. None of the isolates had similar RAPD profi le. Interpretation & conclusions: Our fi ndings showed lactobacilli species present in healthy vagina of women in India differ from those reported from other countries. This information would be useful to development of probiotic tablets seeking to replenish the missing lactobacilli for reproductive health of women in India.en_US
dc.identifier.citationGarg Kavita Bansal, Ganguli Indrani, Das Ram, Talwar G P. Spectrum of Lactobacillus species present in healthy vagina of Indian women. Indian Journal of Medical Research. 2009 June; 129(6): 652-657.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/135841
dc.language.isoenen_US
dc.source.urihttps://icmr.nic.in/ijmr/2009/june/0605.pdfen_US
dc.subjectBacterial vaginosisen_US
dc.subjectLactobacillusen_US
dc.subjectPCRen_US
dc.subjectRAPDen_US
dc.subject16S rDNA sequencingen_US
dc.subjectvariable strainsen_US
dc.subject.meshAdolescent
dc.subject.meshAdult
dc.subject.meshBacterial Typing Techniques
dc.subject.meshDNA, Bacterial --analysis
dc.subject.meshFemale
dc.subject.meshHumans
dc.subject.meshIndia
dc.subject.meshLactobacillus --classification
dc.subject.meshLactobacillus --genetics
dc.subject.meshLactobacillus --isolation & purification
dc.subject.meshMiddle Aged
dc.subject.meshMolecular Sequence Data
dc.subject.meshProbiotics --therapeutic use
dc.subject.meshRNA, Ribosomal, 16S --genetics
dc.subject.meshVagina --microbiology
dc.subject.meshVaginosis, Bacterial --microbiology
dc.subject.meshVaginosis, Bacterial --therapy
dc.subject.meshYoung Adult
dc.titleSpectrum of Lactobacillus species present in healthy vagina of Indian women.en_US
dc.typeArticleen_US
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