Comprehensive analysis of ALK, ROS1 and RET rearrangements in locally advanced rectal cancer

Simple item page
dc.contributor.authorZhou, Chunlianen_US
dc.contributor.authorLi, Minen_US
dc.contributor.authorGuo, Zhiweien_US
dc.contributor.authorKun, Lien_US
dc.contributor.authorXiangming, Zhaien_US
dc.contributor.authorYingchao, Xieen_US
dc.contributor.authorXuexi, Yangen_US
dc.contributor.authorYingsong, Wuen_US
dc.contributor.authorXiao, Weiweien_US
dc.contributor.authorXu, Weiwenen_US
dc.date.accessioned2020-11-18T10:21:10Z
dc.date.available2020-11-18T10:21:10Z
dc.date.issued2020-10
dc.description.abstractGene rearrangements, such as anaplastic lymphoma kinase (ALK), c-ros oncogene 1 receptor tyrosine kinase (ROS1), rearranged during transfection (RET) and neurotrophic receptor tyrosine kinase 1 (NTRK1), identified in cancer have been indicated to be robust therapeutic targets in lung carcinomas. However, a few studies have focussed on locally advanced rectal cancer (LARC). The discovery of novel gene fusions is also valuable for LARC research. We used mass spectrometry-based assays and RNA sequencing to detect both known ALK, ROS1, RET and NTRK1 rearrangements and novel gene fusions in LARC patients. FusionMap was also used to find gene fusions. None of the ALK, ROS1, RET or NTRK1 gene fusions were detected by mass spectrometry-based assays or RNA sequencing. Three fusion candidates, integrin subunit beta 7 (ITGB7)-ROS1, lamin A/C (LMNA)-NTRK1 and Golgi-associated PDZ and coiled-coil motif containing (GOPC)-keratin 8 (KRT8), showed relatively high junction-spanning reads by the FusionMap algorithm, but did not pass validation. These results suggest that no ALK, ROS1 or RET rearrangements were found in LARC.en_US
dc.identifier.affiliationsInstitute of Antibody Engineering, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou 510515, People’s Republic of Chinaen_US
dc.identifier.affiliationsInstitute of Antibody Engineering, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou 510515, People’s Republic of Chinaen_US
dc.identifier.affiliationsDepartment of Radiation Oncology, Sun Yat-sen University Cancer Center, Guangzhou 510060en_US
dc.identifier.affiliationsInstitute of Antibody Engineering, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou 510515, People’s Republic of Chinaen_US
dc.identifier.affiliationsInstitute of Antibody Engineering, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou 510515, People’s Republic of Chinaen_US
dc.identifier.affiliationsInstitute of Antibody Engineering, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou 510515, People’s Republic of Chinaen_US
dc.identifier.citationZhou Chunlian, Li Min, Guo Zhiwei, Kun Li, Xiangming Zhai, Yingchao Xie, Xuexi Yang, Yingsong Wu, Xiao Weiwei, Xu Weiwen. Comprehensive analysis of ALK, ROS1 and RET rearrangements in locally advanced rectal cancer. Journal of Genetics. 2020 Oct; 99: 1-6en_US
dc.identifier.issn0022-1333
dc.identifier.issn0973-7731
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/215550
dc.languageenen_US
dc.publisherIndian Academy of Sciencesen_US
dc.relation.volume99en_US
dc.source.urihttps://doi.org/10.1007/s12041-020-01239-1en_US
dc.subjectrectal neoplasmsen_US
dc.subjectneoadjuvant therapyen_US
dc.subjectRNA sequencingen_US
dc.subjectmass spectrometryen_US
dc.titleComprehensive analysis of ALK, ROS1 and RET rearrangements in locally advanced rectal canceren_US
dc.typeJournal Articleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
jmsh2019v99pf1.pdf
Size:
344.48 KB
Format:
Adobe Portable Document Format
Download
Collections
Journal of Genetics