Effect of relative centrifugal forces and centrifugation times on recovery of Mycobacterium tuberculosis.

Simple item page
dc.contributor.authorSharma, A
dc.contributor.authorMadaan, I
dc.contributor.authorShinu, P
dc.contributor.authorSingh, VA
dc.contributor.authorGarg, A
dc.date.accessioned2016-11-09T05:53:30Z
dc.date.available2016-11-09T05:53:30Z
dc.date.issued2014-01
dc.description.abstractBackground: Tuberculosis (TB) is one of the major cause of morbidity and mortality worldwide, affecting one third of world’s population and the incidence is much higher in South-East Asia (India and China together account for nearly 40% of the global TB cases) Objectives: The current study was designed to evaluate the efficacy of relative centrifugal forces (RCFs) and various centrifugations times (CTs) on recovery of Mycobacterium tuberculosis (MTB) from sputum specimens pre-inoculated with MTB. Materials & Methods: For this study, direct acid fast bacilli (AFB) smear negative sputum specimens were randomly collected, which were further subjected to sterilization ( by autoclaving at 1210 C for 15 min ) and then seeded with MTB. Afterwards, these sputum specimens were subjected to centrifugation (in a cold centrifuge) at various RCFs and CTs. After centrifugation, supernatants and sediments were inoculated on LJ media and incubated at 370 C with daily monitoring for 8 weeks. Results: In the current study, as the RCFs (6000xg) and CTs ( 25 min and 20 min) were increased, the growth detection time exponentially decreased (9 and 11 days) and culture grades (4+) were increased when cultured from the sediments. However, the recovery rates of MTB from the supernatant fluids were constantly decreased as RCFs and CTs increased. Conclusion: The current study concluded that a higher RCF of 6000xg and CTs of 20 and 25 min could effectively detect the tubercle bacilli from the sputum specimens more efficiently than other lower RCFs and CTs.en_US
dc.identifier.citationSharma A, Madaan I, Shinu P, Singh VA, Garg A. Effect of relative centrifugal forces and centrifugation times on recovery of Mycobacterium tuberculosis. International Journal of Medical and Dental Sciences. 2014 Jan; 3(1): 257-263.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/178332
dc.language.isoenen_US
dc.source.urihttps://ijmds.org/wp-content/uploads/2014/10/ijmds.org_257-263-RA-Effect-of-RCF-CT-on-recovery.pdfen_US
dc.subjectPulmonary tuberculosisen_US
dc.subjectMycobacterium tuberculosisen_US
dc.subjectacid fast bacillien_US
dc.subjectrelative centrifugal forceen_US
dc.subjectcentrifugation timeen_US
dc.titleEffect of relative centrifugal forces and centrifugation times on recovery of Mycobacterium tuberculosis.en_US
dc.typeArticleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
ijmds2014v3n1p257.pdf
Size:
381.83 KB
Format:
Adobe Portable Document Format
Description:
Research article
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
International Journal of Medical and Dental Sciences