Susceptibility of lactase to luminal proteases in developing rat intestine.

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dc.contributor.authorKaur, Kamaljiten_US
dc.contributor.authorMahmood, Safrunen_US
dc.contributor.authorMahmood, Akhtaren_US
dc.date.accessioned2006-07-16en_US
dc.date.accessioned2009-05-29T03:22:12Z
dc.date.available2006-07-16en_US
dc.date.available2009-05-29T03:22:12Z
dc.date.issued2006-07-16en_US
dc.description.abstractBACKGROUND: Postnatal development of rat intestine is associated with a decline in brush-border lactase activity. This phenomenon is similar to the adulthood hypolactasia in humans. However, the mechanism underlying this process is not understood. METHODS: The effect of luminal proteases from adult rat intestine on the intestinal lactase activity in animals aged 7, 14, 21 and 30 days was studied in in vitro experiments. Lactase levels were estimated using enzyme assays and Western blot analysis. RESULTS: Incubation of purified brush borders with increasing concentrations of luminal proteases reduced the lactase activity in intestine of 7-day-old rats, but not in that of adult animals. Western blot analysis revealed low signal of the 220-kDa lactase protein in 7-day-old animals, but not that of older weaned animals. CONCLUSIONS: Our findings suggest that luminal proteases may be responsible for the maturational decline in intestinal lactase activity.en_US
dc.description.affiliationDepartment of Experimental Medicine and Biotechnology, Postgraduate Institute of Medical Education and Research, Chandigarh 160 012, India.en_US
dc.identifier.citationKaur K, Mahmood S, Mahmood A. Susceptibility of lactase to luminal proteases in developing rat intestine. Indian Journal of Gastroenterology. 2006 Jul-Aug; 25(4): 179-81en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/65254
dc.language.isoengen_US
dc.source.urihttps://www.indianjgastro.comen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAnimals, Newbornen_US
dc.subject.meshBlotting, Westernen_US
dc.subject.meshEndopeptidases --pharmacologyen_US
dc.subject.meshIntestines --drug effectsen_US
dc.subject.meshLactase --drug effectsen_US
dc.subject.meshMaleen_US
dc.subject.meshMicrovilli --drug effectsen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Wistaren_US
dc.titleSusceptibility of lactase to luminal proteases in developing rat intestine.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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