Epitope analysis of strains of Japanese encephalitis virus by monoclonal antibodies.

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dc.contributor.authorGhosh, S Nen_US
dc.contributor.authorSathe, P Sen_US
dc.contributor.authorSarthi, S Aen_US
dc.contributor.authorCecilia, Den_US
dc.contributor.authorDandawate, C Nen_US
dc.contributor.authorAthawale, S Sen_US
dc.contributor.authorPant, Uen_US
dc.date.accessioned1989-11-01en_US
dc.date.accessioned2009-05-27T08:31:20Z
dc.date.available1989-11-01en_US
dc.date.available2009-05-27T08:31:20Z
dc.date.issued1989-11-01en_US
dc.description.abstractTwenty one strains of Japanese encephalitis (JE) virus, including 16 from India, were compared antigenically on the basis of their reactivity in immunofluorescence (IF), haemagglutination inhibition (HI), ELISA with captured antigen (ECA), and neutralization (N) tests with JE monoclonal antibodies (MAbs). These MAbs represented three domains of distinct epitopes on the envelope protein, designated as Hs-1 to 4 (JE specific in HI), Hx-1 to 5 (flavivirus cross reactive in HI) and NHs-1 to 2 (non-HI JE virus specific). Fifteen of the 21 strains studied were placed in group I. These reacted with MAbs representing the three domains in all the tests indicating presence of the three types of epitopes with full functional activity. The remaining six strains were placed in group II and showed loss in HI reactivity with Hs MAbs but not with Hx MAbs. All the group II strains also reacted in IF and ECA with NHs-1. Hs epitopes in three strains, G9473 (Tamil Nadu), 641686 (Tamil Nadu) and 822199 (Karnataka), appeared to have mutated partially, indicating loss in HI reactivity with Hs MAbs only, while there was retention of other reactivities, viz., IF, ECA and to some extent N test with G9473 and 641686. The remaining three strains, 691004 (Sri Lankan), 755468 (West Bengal) and Yoken (Japan) of group II showed almost complete loss of Hs-1 and Hs-2 epitopes as there was absence of reactivity in IF, ECA and N test in addition to HI. However, Hs-3 MAb showed reactivity in IF with these strains.(ABSTRACT TRUNCATED AT 250 WORDS)en_US
dc.identifier.citationGhosh SN, Sathe PS, Sarthi SA, Cecilia D, Dandawate CN, Athawale SS, Pant U. Epitope analysis of strains of Japanese encephalitis virus by monoclonal antibodies. Indian Journal of Medical Research. 1989 Nov; 89(): 368-75en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/24740
dc.language.isoengen_US
dc.source.urihttps://icmr.nic.in/ijmr/ijmr.htmen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntibodies, Monoclonal --immunologyen_US
dc.subject.meshAntigenic Variationen_US
dc.subject.meshAntigens, Viral --analysisen_US
dc.subject.meshEncephalitis Virus, Japanese --immunologyen_US
dc.subject.meshEpitopes --analysisen_US
dc.subject.meshHumansen_US
dc.titleEpitope analysis of strains of Japanese encephalitis virus by monoclonal antibodies.en_US
dc.typeComparative Studyen_US
dc.typeJournal Articleen_US
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