Electron microscopic studies on Vibrio cholerae O139.

dc.contributor.authorGarg, Sen_US
dc.contributor.authorGhosh, A Nen_US
dc.contributor.authorMukhopadhyay, A Ken_US
dc.contributor.authorNair, G Ben_US
dc.date.accessioned1996-07-01en_US
dc.date.accessioned2009-05-27T06:28:04Z
dc.date.available1996-07-01en_US
dc.date.available2009-05-27T06:28:04Z
dc.date.issued1996-07-01en_US
dc.description.abstractWe conducted studies to investigate the surface architecture of V. cholerae O139 using electron microscopy and compared it with O1 and other serogroups of V. cholerae. The bacterium is comma-shaped and has a single polar flagellum and morphologically resembles the classical and E1Tor biotypes of V. cholerae O1. High power electron microscopy showed a few pili, 5 to 7 nm in diameter, and 2 to 3 in number per bacterium. The presence of a capsule on electron microscopy of ultrathin sections of V. cholerae O139 treated with polycationic ferritin clearly distinguished the O139 serogroup from the O1 serogroup which are not encapsulated. Immunoelectron microscopy further revealed that an anti-O139 monoclonal antibody of the IgG2a isotype bound specifically only to an O139 strain but not to any other serogroup of V. cholerae indicating that O139 has unique epitopes not found in other serogroups of V. cholerae.en_US
dc.description.affiliationNational Institute of Cholera & Enteric Diseases, Calcutta.en_US
dc.identifier.citationGarg S, Ghosh AN, Mukhopadhyay AK, Nair GB. Electron microscopic studies on Vibrio cholerae O139. Indian Journal of Medical Research. 1996 Jul; 104(): 134-8en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/16375
dc.language.isoengen_US
dc.source.urihttps://icmr.nic.in/ijmr/ijmr.htmen_US
dc.subject.meshBacterial Capsulesen_US
dc.subject.meshMicroscopy, Electronen_US
dc.subject.meshVibrio cholerae --ultrastructureen_US
dc.titleElectron microscopic studies on Vibrio cholerae O139.en_US
dc.typeJournal Articleen_US
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