Peptidyl transferase activity of tRNA: a quantum chemical study.

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dc.contributor.authorBhattacharyya, Den_US
dc.contributor.authorDas, G Ken_US
dc.contributor.authorBurma, D Pen_US
dc.date.accessioned2001-02-21en_US
dc.date.accessioned2009-05-27T09:27:08Z
dc.date.available2001-02-21en_US
dc.date.available2009-05-27T09:27:08Z
dc.date.issued2001-02-21en_US
dc.description.abstractThe mechanism of protein synthesis is still unknown due to inability to detect the so-called enzyme "peptidyl transferase" even after elucidation of high-resolution crystal structure of ribosome. We have recently shown by model building and semi-empirical energy calculation that the tRNA molecule at P-site of ribosome may act as peptidyl transferase (Das et al. (1999) J. Theor. Biol. 200, 193-205). We proposed that the tetrahedral intermediate formed from nucleophylic attack of CO of P-site amino-acylated tRNA by NH2 of A-site amino-acylated tRNA is converted to a six-member ring intermediate by conformational change. This ring intermediate produces a free tRNA and a tRNA covalently linked to a peptide. However, energy of the six-member ring intermediate was calculated to be quite high. We show here that the energy values of all the reactants, intermediates and products are within the expected range when they are calculated using high level ab initio quantum chemical methods.en_US
dc.description.affiliationBiophysics Division, Saha Institute of Nuclear Physics, 37 Belgachia Road, Calcutta 700037, India. bhatta@biop.saha.ernet.inen_US
dc.identifier.citationBhattacharyya D, Das GK, Burma DP. Peptidyl transferase activity of tRNA: a quantum chemical study. Indian Journal of Biochemistry & Biophysics. 2001 Feb-Apr; 38(1-2): 48-52en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/27549
dc.language.isoengen_US
dc.source.urihttps://https://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijbb/ijbb0.aspen_US
dc.subject.meshAlanine --chemistryen_US
dc.subject.meshBinding Sitesen_US
dc.subject.meshKineticsen_US
dc.subject.meshModels, Chemicalen_US
dc.subject.meshPeptides --chemistryen_US
dc.subject.meshPeptidyl Transferases --metabolismen_US
dc.subject.meshProtein Structure, Tertiaryen_US
dc.subject.meshRNA, Transfer --chemistryen_US
dc.subject.meshRibose --chemistryen_US
dc.subject.meshThermodynamicsen_US
dc.titlePeptidyl transferase activity of tRNA: a quantum chemical study.en_US
dc.typeJournal Articleen_US
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