Growth modulation of fibroblasts by chitosan-polyvinyl pyrrolidone hydrogel: implications for wound management?

Simple item page
dc.contributor.authorRisbud, Men_US
dc.contributor.authorHardikar, Aen_US
dc.contributor.authorBhonde, Ren_US
dc.date.accessioned2000-03-29en_US
dc.date.accessioned2009-06-01T14:49:37Z
dc.date.available2000-03-29en_US
dc.date.available2009-06-01T14:49:37Z
dc.date.issued2000-03-29en_US
dc.description.abstractWounds in adults and fetuses differ in their healing ability with respect to scar formation. In adults, wounds lacking the epidermis exhibit excess collagen production and scar formation. Fibroblasts synthesize and deposit a collagen rich extracellular matrix. The early migration and proliferation of fibroblasts in the wound area is implicated in wound scarring. We have synthesized a hydrogel from chitosan-polyvinyl pyrrolidone (PVP) and examined its effect on fibroblast growth modulation in vitro. The hydrogel was found to be hydrophilic as seen from its octane contact angle (141.2+/-0.37 degrees). The hydrogel was non-toxic and biocompatible with fibroblasts and epithelial cells as confirmed by the 3(4,5-dimethylthiazolyl-2)-2, 5-diphenyl tetrazolium bromide (MTT) as-say. It showed dual properties by supporting growth of epithelial cells (SiHa) and selectively inhibiting fibro-blast (NIH3T3) growth. Growth inhibition of fibroblasts resulted from their inability to attach on to the hydrogel. These findings are supported by image analysis, which revealed a significant difference (P<0.05) between the number of fibroblasts attached to the hydrogel in tissue culture as compared to tissue culture treated polystyrene (TCPS) controls. However, no significant difference was observed (P>0.05) in the number of epithelial (SiHa) cells attached on to the hydrogel as compared to the TCPS control. Although in vivo experiments are awaited, these findings point to the possible use of chitosan-PVP hydrogels in wound-management.en_US
dc.description.affiliationTissue Engineering and Banking Laboratory, National Centre for Cell Science, Ganeshkhind, Pune 411 007, India. mrisbud@nccs.res.inen_US
dc.identifier.citationRisbud M, Hardikar A, Bhonde R. Growth modulation of fibroblasts by chitosan-polyvinyl pyrrolidone hydrogel: implications for wound management? Journal of Biosciences. 2000 Mar; 25(1): 25-31en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/111105
dc.language.isoengen_US
dc.source.urihttps://www.ias.ac.in/jbiosci/index.htmlen_US
dc.subject.mesh3T3 Cellsen_US
dc.subject.meshAnimalsen_US
dc.subject.meshChitin --analogs & derivativesen_US
dc.subject.meshChitosanen_US
dc.subject.meshFibroblasts --cytologyen_US
dc.subject.meshHydrogels --therapeutic useen_US
dc.subject.meshImage Processing, Computer-Assisteden_US
dc.subject.meshMiceen_US
dc.subject.meshPovidone --analogs & derivativesen_US
dc.subject.meshWound Healing --drug effectsen_US
dc.titleGrowth modulation of fibroblasts by chitosan-polyvinyl pyrrolidone hydrogel: implications for wound management?en_US
dc.typeJournal Articleen_US
Files
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.79 KB
Format:
Plain Text
Description:
Download
Collections
Journal of Biosciences