Overview of flavivirus molecular biology and future vaccine development via recombinant DNA.

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dc.contributor.authorRice, C Men_US
dc.date.accessioned2009-05-27T16:09:46Z
dc.date.available2009-05-27T16:09:46Z
dc.date.issued1990-12-01en_US
dc.descriptionThe Southeast Asian Journal of Tropical Medicine and Public Health. 54 references.en_US
dc.description.abstractStudies in many laboratories over the last several years have elucidated the structures of several different flavivirus genomes. Conserved features include the production of at least 10 different virus encoded proteins from a single long open reading frame by a combination of host and virus-encoded proteases. The established gene order is 5'-C- prM(M)-E-NS1-NS2A-NS2B-NS3-NS4A-NS4B-NS 5-3' and these proteins exhibit varying degrees of homology in comparisons among flaviviruses. Conserved RNA sequences and structures have also been identified for the mosquito-borne flaviviruses but are absent in sequenced tick-bone viruses. Relevant to the development of efficacious flavivirus vaccines, studies aimed at defining the antigenic determinants necessary for eliciting protective immunity have focused primarily on the structural proteins, in particular the E protein, as well as the nonstructural secreted glycoprotein, NS1. Other work, which has led to the derivation of live-attenuated flavivirus strains, should eventually allow the genetic determinants of flavivirus attenuation and pathogenesis to be understood at the molecular level. The successful recovery infectious flaviviruses from cloned cDNA raises the possibility of manipulating these viral genomes as cDNA to construct or propagate candidate live-attenuated vaccine strains. Several applications of this technology are discussed.en_US
dc.description.affiliationDepartment of Molecular Microbiology, Washington University School of Medicine, St Louis, Missouri 63110-1093.en_US
dc.identifier.citationRice CM. Overview of flavivirus molecular biology and future vaccine development via recombinant DNA. The Southeast Asian Journal of Tropical Medicine and Public Health. 1990 Dec; 21(4): 670-7en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/34563
dc.language.isoengen_US
dc.source.urihttps://www.tm.mahidol.ac.th/seameo/publication.htmen_US
dc.subject.meshAmino Acid Sequence --geneticsen_US
dc.subject.meshChromosome Mappingen_US
dc.subject.meshDNA, Viral --geneticsen_US
dc.subject.meshFlavivirus --geneticsen_US
dc.subject.meshForecastingen_US
dc.subject.meshGenes, Viral --geneticsen_US
dc.subject.meshHumansen_US
dc.subject.meshMolecular Biologyen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshRNA, Viral --geneticsen_US
dc.subject.meshVaccines, Synthetic --biosynthesisen_US
dc.titleOverview of flavivirus molecular biology and future vaccine development via recombinant DNA.en_US
dc.typeJournal Articleen_US
dc.typeReviewen_US
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