Quantitation of tetanus and diphtheria antitoxins in mouse sera by indirect haemagglutination.

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dc.contributor.authorMaheshwari, S Cen_US
dc.contributor.authorSharma, S Ben_US
dc.contributor.authorKumar, Aen_US
dc.contributor.authorSokhey, Jen_US
dc.date.accessioned1998-03-08en_US
dc.date.accessioned2009-06-01T18:05:42Z
dc.date.available1998-03-08en_US
dc.date.available2009-06-01T18:05:42Z
dc.date.issued1998-03-08en_US
dc.description.abstractSerum samples obtained from 75 groups of mice immunized with various doses of adsorbed tetanus vaccine, adsorbed diphtheria-tetanus vaccine and adsorbed diphtheria-tetanus-pertussis vaccine were titrated for tetanus antitoxin content by an in-vitro indirect haemagglutination (IHA) and by toxin neutralization test (TN) in mice. From these serum samples of 49 groups of mice which were immunized with combined vaccine containing diphtheria toxoid were titrated for their diphtheria antitoxin content by IHA and by i.d. toxin neutralization test (TN) in guinea pigs. Good correlations were found between the estimates obtained by in-vitro IHA and in vivo TN tests in both tetanus and diphtheria antitoxin titrations. The minimum level of tetanus or diphtheria antitoxin detectable by IHA was 0.00039 IU/ml. It is concluded that IHA is a simple, sensitive and reproducible alternative test which can replace the animal TN tests for the estimation of tetanus and diphtheria antitoxins and could reliably be used in the potency assay of tetanus and diphtheria toxoids of combined vaccines based on antibody induction in mice.en_US
dc.description.affiliationCentral Research Institute, Himachal Pradesh.en_US
dc.identifier.citationMaheshwari SC, Sharma SB, Kumar A, Sokhey J. Quantitation of tetanus and diphtheria antitoxins in mouse sera by indirect haemagglutination. Journal of Communicable Diseases. 1998 Mar; 30(1): 23-8en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/111974
dc.language.isoengen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBiological Assayen_US
dc.subject.meshDiphtheria Antitoxin --blooden_US
dc.subject.meshDiphtheria Toxoid --immunologyen_US
dc.subject.meshDiphtheria-Tetanus-Pertussis Vaccine --immunologyen_US
dc.subject.meshDrug Evaluation, Preclinicalen_US
dc.subject.meshHemagglutination Tests --methodsen_US
dc.subject.meshMiceen_US
dc.subject.meshReproducibility of Resultsen_US
dc.subject.meshSensitivity and Specificityen_US
dc.subject.meshTetanus Antitoxin --blooden_US
dc.subject.meshTetanus Toxoid --immunologyen_US
dc.subject.meshVaccines, Combineden_US
dc.titleQuantitation of tetanus and diphtheria antitoxins in mouse sera by indirect haemagglutination.en_US
dc.typeJournal Articleen_US
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