LipL32, an outer membrane protein of Leptospira, as an antigen in a dipstick assay for diagnosis of leptospirosis.

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dc.contributor.authorBoonyod, Doojdaoen_US
dc.contributor.authorPoovorawan, Yongen_US
dc.contributor.authorBhattarakosol, Parvapanen_US
dc.contributor.authorChirathaworn, Chintanaen_US
dc.date.accessioned2009-05-27T17:09:01Z
dc.date.available2009-05-27T17:09:01Z
dc.date.issued2005-12-29en_US
dc.descriptionPublished by the Allergy and Immunology Society of Thailand.en_US
dc.description.abstractMicroscopic agglutination test (MAT), as well as other serological assays that aimed at detecting antibodies to Leptospira, supplements the leptospirosis diagnosis based on the clinical features. Nevertheless, false positive results have been occasionally reported when the crude antigen was used in those antibody-based tests due either to the presence of antibodies stimulated by other antigenically related pathogens in the patient's serum, or the antibodies in the serum may be stimulated by a previously unrecognized Leptospira infection, especially in the disease endemic areas. Thus, the more refined antigen should improve the serodiagnostic accuracy. Among Leptospira spp., LipL32, which is a pathogenic Leptospira outer membrane protein (OMP), expressed by the bacteria grown both in vitro and in vivo. In this study, recombinant LipL32 protein was tested by a dipstick method for its potential in serodiagnosis of leptospirosis. Preliminary results suggest that the recombinant LipL32 is a good diagnostic detection reagent for specific Leptospira IgG. Diagnostic sensitivity and specificity of the Lip32 dipstick assay, when compared to those of MAT, were 100% and 98.33%, respectively.en_US
dc.description.affiliationInter-Department of Medical Microbiology, Faculty of Graduate School, Chulalongkorn University, Bangkok, Thailand.en_US
dc.identifier.citationBoonyod D, Poovorawan Y, Bhattarakosol P, Chirathaworn C. LipL32, an outer membrane protein of Leptospira, as an antigen in a dipstick assay for diagnosis of leptospirosis. Asian Pacific Journal of Allergy and Immunology. 2005 Jun-Sep; 23(2-3): 133-41en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/36560
dc.language.isoengen_US
dc.subject.meshAgglutination Testsen_US
dc.subject.meshAntibodies, Bacterial --blooden_US
dc.subject.meshAntigens, Bacterial --blooden_US
dc.subject.meshBacterial Outer Membrane Proteins --immunologyen_US
dc.subject.meshCase-Control Studiesen_US
dc.subject.meshDNA, Bacterial --isolation & purificationen_US
dc.subject.meshFluorescent Antibody Technique, Indirecten_US
dc.subject.meshGenome, Bacterialen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunoglobulin G --blooden_US
dc.subject.meshImmunoglobulin M --blooden_US
dc.subject.meshLeptospira --geneticsen_US
dc.subject.meshLeptospirosis --blooden_US
dc.subject.meshLipoproteins --immunologyen_US
dc.subject.meshRecombinant Proteins --immunologyen_US
dc.subject.meshSensitivity and Specificityen_US
dc.subject.meshSequence Analysis, DNAen_US
dc.titleLipL32, an outer membrane protein of Leptospira, as an antigen in a dipstick assay for diagnosis of leptospirosis.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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