Cloning and characterization of a DNA repair gene in Haemophilus influenzae.
| dc.contributor.author | Kanade, Rohinee P | |
| dc.contributor.author | Notani, N K | |
| dc.date.accessioned | 2015-07-25T05:57:19Z | |
| dc.date.available | 2015-07-25T05:57:19Z | |
| dc.date.issued | 1987-06 | |
| dc.description.abstract | Using a high-efficiency DNA cloning vector pJ1-8, a DNA repair gene uvr1 has been self-cloned in bacterium Haemophilus influenzae. Chimeric plasmid pKuvrl, carrying wild type allele of uvr1 gene and flanking DNA sequences, specifically complements a uvrl gene mutation in the bacterial chromosome. A uvr1– mutation could be transferred from chromosome by in vivo recombination to pKuvr1 and isolated and designated as plasmid pKuvrl –. Plasmid pKuvrl carries a 11·3 kb chromosomal DNA insert which was scanned for the presence of any other DNA repair genes by a novel method of directed mutagenesis. Preliminary analysis of the 3 new mutants isolated by this method supports the notion that the insert contains more than one gene concerned with ultraviolet radiation-sensitivity. | en_US |
| dc.identifier.citation | Kanade Rohinee P, Notani N K. Cloning and characterization of a DNA repair gene in Haemophilus influenzae. Journal of Biosciences. 1987 Jun; 12(2): 115-123. | en_US |
| dc.identifier.uri | https://imsear.searo.who.int/handle/123456789/160569 | |
| dc.language.iso | en | en_US |
| dc.source.uri | https://www.ias.ac.in/jarch/jbiosci/12/115-123.pdf | en_US |
| dc.subject | Haemophilus influenzae | en_US |
| dc.subject | self-cloning | en_US |
| dc.subject | uvr1 gene | en_US |
| dc.subject | directed mutagenesis | en_US |
| dc.title | Cloning and characterization of a DNA repair gene in Haemophilus influenzae. | en_US |
| dc.type | Article | en_US |