Sphingosine-1 phosphate prevents ethanol-induced corneal epithelial apoptosis.

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dc.contributor.authorFournie, Pierre
dc.contributor.authorGaliacy, Stéphane
dc.contributor.authorRanty, Marie-Laure
dc.contributor.authorRico-Lattes, Isabelle
dc.contributor.authorMalecaze, François
dc.contributor.authorQuintyn, Jean-Claude
dc.date.accessioned2012-06-01T08:45:03Z
dc.date.available2012-06-01T08:45:03Z
dc.date.issued2012-03
dc.description.abstractBackground: Apoptosis is a programmed cell death in multicellular organisms, found in a wide variety of conditions, including inflammatory process, everywhere in the body, including the cornea and conjunctiva. Aim: To evaluate the effect of a new topical formulation of sphingosine-1 phosphate on preventing apoptosis of the corneal epithelium. Setting: Medical University. Materials and Methods: We tested several formulations suitable for topical application. Twenty-five rabbits were distributed among five groups. Group 1 comprised the controls. In Group 2, 20% ethanol was applied topically for 20 seconds; in Group 3, 50 μM topical sphingosine-1 phosphate was applied 2 hours prior to 20% ethanol application. In Group 4, 200 μM topical sphingosine-1 phosphate was applied 2 hours before the 20% ethanol application. In Group 5, only 200 μM topical sphingosine-1 phosphate was applied. Apoptosis was evaluated using the terminal deoxynucleotidyl transferase biotin-dUTP Nick End Labeling (TUNEL) assay. Pairwise comparisons were performed using t-tests with Scheffe's correction. Data were analyzed using STATA 9.0 statistical software. Results: A suspension of sphingosine-1 phosphate in the presence of Montanox 80 was stable and could be formulated without sonication. Epithelial apoptosis was detected only in Groups 2 and 3. Conclusion: Sphingosine-1 phosphate can prevent ethanol-induced apoptosis in the corneal epithelium of rabbits.en_US
dc.identifier.citationFournie Pierre, Galiacy Stéphane, Ranty Marie-Laure, Rico-Lattes Isabelle, Malecaze François, Quintyn Jean-Claude. Sphingosine-1 phosphate prevents ethanol-induced corneal epithelial apoptosis. Indian Journal of Ophthalmology. 2012 Mar; 60(2): 115-118.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/138804
dc.language.isoenen_US
dc.source.urihttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3339070/en_US
dc.subjectApoptosisen_US
dc.subjectcorneal epitheliumen_US
dc.subjectformulationen_US
dc.subjectsphingosine-1 phosphateen_US
dc.subject.meshAnimals
dc.subject.meshAnti-Infective Agents, Local --toxicity
dc.subject.meshApoptosis --drug effects
dc.subject.meshCorneal Diseases --chemically induced
dc.subject.meshCorneal Diseases --pathology
dc.subject.meshCorneal Diseases --prevention & control
dc.subject.meshDisease Models, Animal
dc.subject.meshEpithelium, Corneal --drug effects
dc.subject.meshEthanol --toxicity
dc.subject.meshLysophospholipids --pharmacology
dc.subject.meshRabbits
dc.subject.meshSphingosine --analogs & derivatives
dc.subject.meshSphingosine --pharmacology
dc.titleSphingosine-1 phosphate prevents ethanol-induced corneal epithelial apoptosis.en_US
dc.typeArticleen_US
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