Sphingosine-1 phosphate prevents ethanol-induced corneal epithelial apoptosis.
dc.contributor.author | Fournie, Pierre | |
dc.contributor.author | Galiacy, Stéphane | |
dc.contributor.author | Ranty, Marie-Laure | |
dc.contributor.author | Rico-Lattes, Isabelle | |
dc.contributor.author | Malecaze, François | |
dc.contributor.author | Quintyn, Jean-Claude | |
dc.date.accessioned | 2012-06-01T08:45:03Z | |
dc.date.available | 2012-06-01T08:45:03Z | |
dc.date.issued | 2012-03 | |
dc.description.abstract | Background: Apoptosis is a programmed cell death in multicellular organisms, found in a wide variety of conditions, including inflammatory process, everywhere in the body, including the cornea and conjunctiva. Aim: To evaluate the effect of a new topical formulation of sphingosine-1 phosphate on preventing apoptosis of the corneal epithelium. Setting: Medical University. Materials and Methods: We tested several formulations suitable for topical application. Twenty-five rabbits were distributed among five groups. Group 1 comprised the controls. In Group 2, 20% ethanol was applied topically for 20 seconds; in Group 3, 50 μM topical sphingosine-1 phosphate was applied 2 hours prior to 20% ethanol application. In Group 4, 200 μM topical sphingosine-1 phosphate was applied 2 hours before the 20% ethanol application. In Group 5, only 200 μM topical sphingosine-1 phosphate was applied. Apoptosis was evaluated using the terminal deoxynucleotidyl transferase biotin-dUTP Nick End Labeling (TUNEL) assay. Pairwise comparisons were performed using t-tests with Scheffe's correction. Data were analyzed using STATA 9.0 statistical software. Results: A suspension of sphingosine-1 phosphate in the presence of Montanox 80 was stable and could be formulated without sonication. Epithelial apoptosis was detected only in Groups 2 and 3. Conclusion: Sphingosine-1 phosphate can prevent ethanol-induced apoptosis in the corneal epithelium of rabbits. | en_US |
dc.identifier.citation | Fournie Pierre, Galiacy Stéphane, Ranty Marie-Laure, Rico-Lattes Isabelle, Malecaze François, Quintyn Jean-Claude. Sphingosine-1 phosphate prevents ethanol-induced corneal epithelial apoptosis. Indian Journal of Ophthalmology. 2012 Mar; 60(2): 115-118. | en_US |
dc.identifier.uri | https://imsear.searo.who.int/handle/123456789/138804 | |
dc.language.iso | en | en_US |
dc.source.uri | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3339070/ | en_US |
dc.subject | Apoptosis | en_US |
dc.subject | corneal epithelium | en_US |
dc.subject | formulation | en_US |
dc.subject | sphingosine-1 phosphate | en_US |
dc.subject.mesh | Animals | |
dc.subject.mesh | Anti-Infective Agents, Local --toxicity | |
dc.subject.mesh | Apoptosis --drug effects | |
dc.subject.mesh | Corneal Diseases --chemically induced | |
dc.subject.mesh | Corneal Diseases --pathology | |
dc.subject.mesh | Corneal Diseases --prevention & control | |
dc.subject.mesh | Disease Models, Animal | |
dc.subject.mesh | Epithelium, Corneal --drug effects | |
dc.subject.mesh | Ethanol --toxicity | |
dc.subject.mesh | Lysophospholipids --pharmacology | |
dc.subject.mesh | Rabbits | |
dc.subject.mesh | Sphingosine --analogs & derivatives | |
dc.subject.mesh | Sphingosine --pharmacology | |
dc.title | Sphingosine-1 phosphate prevents ethanol-induced corneal epithelial apoptosis. | en_US |
dc.type | Article | en_US |