Differential response of cytokines induced by Leptospira interrogans, serogroup Pomona, serovar Pomona, in mouse and human cell lines.

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dc.contributor.authorJongyota, Woranarten_US
dc.contributor.authorWigraipat, Chantiwaen_US
dc.contributor.authorNontapa, Suwannaen_US
dc.contributor.authorTaweechaisupapong, Suwimolen_US
dc.contributor.authorWara-Aswapati, Nawarat Cen_US
dc.contributor.authorWongratanacheewin, Surasakdien_US
dc.contributor.authorSermswan, Rasana Wen_US
dc.date.accessioned2009-05-27T17:25:03Z
dc.date.available2009-05-27T17:25:03Z
dc.date.issued2008-12-26en_US
dc.descriptionPublished by the Allergy and Immunology Society of Thailand.en_US
dc.description.abstractLeptospira interrogans, the causative agent of leptospirosis, is an important zoonotic bacterium. The mechanisms and roles of cytokine induction in both humans and animals remain unclear. Therefore, the IFN-gamma, IL-6, IL-10 and IL-12 levels were measured by enzyme-linked immunosorbent assay (ELISA) in human THP-1 and mouse RAW 264.7 monocyte cell lines following stimulation with heat-killed Leptospira interrogans serogroup Pomona serovar Pomona, L. biflexa, E. coli or Salmonella group B. The production of IL-6 and IL-12 were higher and rose more rapidly in the RAW 264.7 cells with all bacteria. The IL-10 was not detected in the RAW 264.7 cells when induced by leptospires. The IFN-gamma level in human peripheral blood mononuclear cells (PBMCs) induced by leptospires was also significantly lower than with other bacteria. When IL-10 and IL-12 mRNA expressions were detected in hamster's spleen, their patterns were similar to what was observed in THP-1 in that IL-12 was only slightly increased while IL-10 expression was high. Moreover, the IFN-gamma expression could not be detected in hamsters. The more potent cytokine responses in the RAW 264.7 cells may indirectly reflect the disease outcome in mice which render them to be a good reservoir of leptospirosis. Whether these cytokines have contributed to immunoprotection during the L. interrogans infection remains to be further investigated.en_US
dc.description.affiliationDepartment of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, Thailand.en_US
dc.identifier.citationJongyota W, Wigraipat C, Nontapa S, Taweechaisupapong S, Wara-Aswapati NC, Wongratanacheewin S, Sermswan RW. Differential response of cytokines induced by Leptospira interrogans, serogroup Pomona, serovar Pomona, in mouse and human cell lines. Asian Pacific Journal of Allergy and Immunology. 2008 Dec; 26(4): 229-36en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/37146
dc.language.isoengen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCell Lineen_US
dc.subject.meshCytokines --biosynthesisen_US
dc.subject.meshHumansen_US
dc.subject.meshInterferon-gamma --biosynthesisen_US
dc.subject.meshInterleukin-10 --biosynthesisen_US
dc.subject.meshInterleukin-12 --biosynthesisen_US
dc.subject.meshInterleukin-6 --biosynthesisen_US
dc.subject.meshLeptospira interrogans serovar pomona --immunologyen_US
dc.subject.meshLeptospirosis --immunologyen_US
dc.subject.meshLeukocytes, Mononuclear --immunologyen_US
dc.subject.meshMiceen_US
dc.titleDifferential response of cytokines induced by Leptospira interrogans, serogroup Pomona, serovar Pomona, in mouse and human cell lines.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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