Use Of Metabolically Competent Human Hepatoma Cells of The Detection Of Mutagens And Antimutagens.

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dc.contributor.authorKnasmuller, Siegfried
dc.contributor.authorParzefall, Wolfram
dc.contributor.authorSanyal, Ratna
dc.contributor.authorEcker, Sonja
dc.contributor.authorSchwab, Christina
dc.contributor.authorUhl, Maria
dc.contributor.authorMersch-Sundermann, Volker
dc.contributor.authorWilliamson, Gary
dc.contributor.authorHietsch, Gerhard
dc.contributor.authorLanger, Theo
dc.contributor.authorDarroudi, Firouz
dc.contributor.authorNatarajan, Adayapalam T
dc.date.accessioned2015-07-16T08:47:40Z
dc.date.available2015-07-16T08:47:40Z
dc.date.issued1998-04
dc.description.abstractThe human hepatoma cell line (Hep G2) has retained the activities of various phase I and phase II enzymes which play a crucial role in the activation/detoxification of genotoxic procarcinogens and reflects the metabolism of such compounds in vivo better than experimental models with metabolically incompetent cells and exogenous activation mixtures. In recent years, methodologies have been developed which enable the detection of genotoxic effects in Hep G2 cells. Appropriate endpoints are the induction of 6-TGr mutants, of micronuclei and of comets (single cell electrophoresis assays). It has been demonstrated that various classes of environmental carcinogens, such as nitrosamines, aflatoxins, aromatic and heterocyclic amines and polycyclic aromatic hydrocarbons can be detected in genotoxicity assays with Hep G2 cells. Furthermore, it has been shown that these assays can distinguish between structurally related carcinogens and non-carcinogens, and positive results have been obtained with rodent carcinogens (such as safrol and hexamethylphosphoramide) which give false negative results in conventional in vitro assays with rat liver homogenates. Hep G2 cells have also been used in antimutagenicity studies and can identify mechanisms not detected in conventional in vitro systems such as induction of detoxifying enzymes, inactivation of endogenously formed DNA- reactive metabolites and intracellular inhibition of activating enzymes.en_US
dc.identifier.citationKnasmuller Siegfried, Parzefall Wolfram, Sanyal Ratna, Ecker Sonja, Schwab Christina, Uhl Maria, Mersch-Sundermann Volker, Williamson Gary, Hietsch Gerhard, Langer Theo, Darroudi Firouz, Natarajan Adayapalam T. Use Of Metabolically Competent Human Hepatoma Cells of The Detection Of Mutagens And Antimutagens. Indian Journal of Human Genetics. 1998 Apr; 4(2): 157-180.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/159863
dc.language.isoenen_US
dc.source.urihttps://www.ijhg.com/article.asp?issn=0971-6866;year=1998;volume=4;issue=2;spage=157;epage=180;aulast=Knasmuller;type=2en_US
dc.subjectMetabolic activationen_US
dc.subjecthuman Hep G2 cellen_US
dc.subjectmutagenicityen_US
dc.subjectantimutangenesen_US
dc.titleUse Of Metabolically Competent Human Hepatoma Cells of The Detection Of Mutagens And Antimutagens.en_US
dc.typeArticleen_US
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