Spectrophotometric and spectrofluorometric studies on interaction of cationic dyes with bacterial capsular polysaccharide.

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dc.contributor.authorMitra, Aen_US
dc.contributor.authorChakraborty, A Ken_US
dc.date.accessioned1998-08-17en_US
dc.date.accessioned2009-05-27T09:35:26Z
dc.date.available1998-08-17en_US
dc.date.available2009-05-27T09:35:26Z
dc.date.issued1998-08-17en_US
dc.description.abstractInteraction of Klebsiella K14 capsular polysaccharide with cationic dyes pinacyanol chloride, acridine orange and phenosafranin has been studied by spectrophotometric and spectrofluorometric techniques. The polymer containing both glucuronic acid and pyruvic acid in its repeating unit behaved as a unique polyelectrolyte. It induced blue shift of the absorption band of pinacyanol chloride indicating strong metachromasy. Stoichiometry of the polyanion and the dye cations in the polymer-dye compound (1:2) indicated that both glucuronic acid and pyruvic acid acted as potential anionic sites for interaction with the cationic dye molecules. The stoichiometry of anionic site (of polyanion): cationic site (of dye) in the polymer dye compound was calculated as 1:1. Interaction of the polymer with acridine orange and phenosafranin dyes studied by fluorescence measurements demonstrated Stern-Volmer type of quenching. Equivalent weight of the polymer was determined by spectrophotometric and spectrofluorometric titrations. From the present studies chromotropic property of the polymer was established.en_US
dc.description.affiliationDepartment of Chemistry, Tripura University, India.en_US
dc.identifier.citationMitra A, Chakraborty AK. Spectrophotometric and spectrofluorometric studies on interaction of cationic dyes with bacterial capsular polysaccharide. Indian Journal of Biochemistry & Biophysics. 1998 Aug; 35(4): 241-6en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/27964
dc.language.isoengen_US
dc.source.urihttps://https://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijbb/ijbb0.aspen_US
dc.subject.meshAcridine Orange --metabolismen_US
dc.subject.meshBinding Sitesen_US
dc.subject.meshCarbocyanines --metabolismen_US
dc.subject.meshColoring Agents --metabolismen_US
dc.subject.meshKlebsiella --chemistryen_US
dc.subject.meshPhenazines --metabolismen_US
dc.subject.meshPolysaccharides, Bacterial --chemistryen_US
dc.subject.meshSpectrometry, Fluorescenceen_US
dc.subject.meshSpectrophotometryen_US
dc.titleSpectrophotometric and spectrofluorometric studies on interaction of cationic dyes with bacterial capsular polysaccharide.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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