SDF-1 promotes metastasis of NSCLC by enhancing chemoattraction of megakaryocytes through the PI3K/Akt signaling pathway

Simple item page
dc.contributor.authorAI, YIGUOen_US
dc.contributor.authorWAN, CHANGHONGen_US
dc.contributor.authorCHEN, ZIJIANen_US
dc.contributor.authorWANG, YANSHENGen_US
dc.contributor.authorZHAO, WENen_US
dc.contributor.authorHUANG, WEIZHEen_US
dc.date.accessioned2024-11-30T11:24:42Z
dc.date.available2024-11-30T11:24:42Z
dc.date.issued2024-05
dc.description.abstractLung cancer (LC) is the leading cause of cancer-associated deaths worldwide, among which non-small-cell lung cancer (NSCLC) accounts for 80%. Stromal cell-derived factor-1 (SDF-1) inhibition results in a significant depletion of NSCLC metastasis. Additionally, SDF-1 is the only natural chemokine known to bind and activate the receptor CXCR4. Thus, we attempted to clarify the molecular mechanism of SDF-1 underlying NSCLC progression. Transwell migration, adhesion, and G-LISA assays were used to assess megakaryocytic chemotaxis in vitro and in vivo in terms of megakaryocytic migration, adherence, and RhoA activation, respectively. Western blotting was used to assess PI3K/Akt-associated protein abundances in MEG-01 cells and primary megakaryocytes under the indicated treatment. A hematology analyzer and flow cytometry were used to assess platelet counts in peripheral blood and newly formed platelet counts in Lewis LC mice under different treatments. Immunochemistry and flow cytometry were used to measure CD41+ megakaryocyte numbers in Lewis LC mouse tissue under different treatments. ELISA was used to measure serum TPO levels, and H&E staining was used to detect NSCLC metastasis. SDF-1 receptor knockdown suppressed megakaryocytic chemotaxis in Lewis LC mice. SDF-1 receptor inhibition suppressed megakaryocytic chemotaxis via the PI3K/Akt pathway. SDF-1 receptor knockdown suppressed CD41+ megakaryocyte numbers in vivo through PI3K/Akt signaling. SDF-1 receptor inhibition suppressed CD41+ megakaryocytes to hinder NSCLC metastasis. SDF-1 facilitates NSCLC metastasis by enhancing the chemoattraction of megakaryocytes via the PI3K/Akt signaling pathway, which may provide a potential new direction for seeking therapeutic plans for NSCLC.en_US
dc.identifier.affiliationsDepartment of Cardiothoracic Surgery, The Second Affiliated Hospital of Shantou University Medical College, Shantou 515041, Guangdong, Chinaen_US
dc.identifier.affiliationsDepartment of Cardiothoracic Surgery, The Second Affiliated Hospital of Shantou University Medical College, Shantou 515041, Guangdong, Chinaen_US
dc.identifier.affiliationsDepartment of Cardiothoracic Surgery, The Second Affiliated Hospital of Shantou University Medical College, Shantou 515041, Guangdong, Chinaen_US
dc.identifier.affiliationsDepartment of Cardiothoracic Surgery, State Key Laboratory of Respiratory Disease, National Clinical Research Centre for Respiratory Disease, Guangzhou Institute of Respiratory Health, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, Guangdong, Chinaen_US
dc.identifier.affiliationsDepartment of Respiratory and Critical Care Medicine, Shenzhen University General Hospital, Shenzhen 518055, Guangdong, Chinaen_US
dc.identifier.affiliationsDepartment of Cardiothoracic Surgery, The Second Affiliated Hospital of Shantou University Medical College, Shantou 515041, Guangdong, Chinaen_US
dc.identifier.citationAI YIGUO, WAN CHANGHONG, CHEN ZIJIAN, WANG YANSHENG, ZHAO WEN, HUANG WEIZHE. SDF-1 promotes metastasis of NSCLC by enhancing chemoattraction of megakaryocytes through the PI3K/Akt signaling pathway. Journal of Biosciences. 2024 May; 49: 1-10en_US
dc.identifier.issn0250-5991
dc.identifier.issn0973-7138
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/237972
dc.languageenen_US
dc.publisherThe Indian Academy of Sciencesen_US
dc.relation.volume49en_US
dc.source.urihttps://doi.org/10.1007/s12038-023-00393-9en_US
dc.subjectMegakaryocyteen_US
dc.subjectnon-small-cell lung canceren_US
dc.subjectPI3K/Akten_US
dc.subjectSDF-1en_US
dc.titleSDF-1 promotes metastasis of NSCLC by enhancing chemoattraction of megakaryocytes through the PI3K/Akt signaling pathwayen_US
dc.typeJournal Articleen_US
Files
Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
jb2024v49p1ai.pdf
Size:
2.17 MB
Format:
Adobe Portable Document Format
Download
Collections
Journal of Biosciences