Anti-inflammatory activity of Talinum fruticosum L. on formalin induced paw edema in albino rats.
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Date
2012-01
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Abstract
Inflammation is a reaction of a living vascularised tissue to an injury. Conventional
or synthetic drugs used in the treatment of inflammatory diseases are inadequate, it
sometimes have serious side effects. So, number of herbal medicines is recommended for the
treatment of inflammation that has no side effects. The present study is aimed to evaluate the
anti inflammatory activity of Talinum fruticosum L on formalin induced paw edema in rats
as for controlling inflammatory disorders. The objectives of the present study are to carry out
phytochemical screening of selected plant drug , to prepare an aqueous extract from Talinum
fruticosum L and to screen the in vivo anti inflammatory effect of Talinum fruticosum L. For
phytochemical screening, the secondary metabolites like alkaloid, flavonoid, tannin, saponin,
quinine were tested using qualitative spot tests. For anti inflammatory activity, wistar albino
rats were used and divided into 6 groups and treated accordingly:Normal control, formalin
induced group (0.1ml/kg bw), formalin+ Talinum fruticosum L (100mg/kg bw), formalin+
Talinum fruticosum L (200mg/kg bw), formalin+ Talinum fruticosum L (300mg/kg bw),
and plant treated (300mg/kg bw).After the experimental period of 15 days, the blood and
tissue samples were collected and biochemical parameter and histpathological studies were
carried out.The phytochemical screening suggests the standardization, identity, purity and of
presence of phytochemicals like saponins, tannins, flavonoids, terpenoids and cardiac
glycosides. Oral administration of formalin to the experimental animals produced reduction in
the levels of SOD,GSH,GPX ,GR, serum protein and total RBC and Hb. The animals
pretreated with Talinum fruticosum L extract at dose levels of 100,200,300mg/kg bw were
significantly increased the levels of the above parameters. A significant increase in the length
of the paw thickness, in the level of serum enzymes (SGOT, SGPT, ALP, CK) and Lipid
peroxide (LPO), in the level of hydroxy proline, hexosamine and leucocytes was noted in
the rats induced with formalin, while these levels were normalized by pretreatment with
Talinum fruticosum L extract. The histopathological studies of edematous sections of
formalin induced rat paw showed loss of cartilage, osteoblast hyperplasia. Talinum
fruticosum L treated formalin induced rats showed moderate reduction in the cartilage and
osteoblast. From the present observation, it is evidenced that Talinum fruticosum L would be
an effective drug for the treatment of inflammatory reactions.
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Keywords
Talinum fruticosum L., Inflammation, Flavonoid, Terpenoid, Hydroxyproline, Hexosamine
Citation
John N Agnel Arul, Shobana G. Anti-inflammatory activity of Talinum fruticosum L. on formalin induced paw edema in albino rats. Journal of Applied Pharmaceutical Science. 2012 Jan; 2(1): 123-127.