Development of a conjoint phage typing & biotyping schema for Salmonella enterica serovar Senftenberg (S. senftenberg) & the correlation of biotypes with phage types.

Kumar, S; Sharma, N C; Singh, S; Bhatia, R; Singh, H

Development of a conjoint phage typing & biotyping schema for Salmonella enterica serovar Senftenberg (S. senftenberg) & the correlation of biotypes with phage types.

Date

1994-12-01

Authors

Abstract

A total of 287 strains of S. senftenberg received from various parts of India during 1969 to 1992 were phage typed using six lysogenic phages. The typability was 90.3 per cent and 14 different phage types could be defined excluding a small group of untypable strains. A biotyping scheme was developed utilising six characters and 13 biotypes could be defined. Stern's glycerol medium proved to be the best discriminatory medium. Diversity indeces of phage typing and biotyping schemes were 0.868 and 0.503 respectively. Better discrimination was obtained when phage types were subdivided into different biotypes with a diversity index of 0.931. The schemes were found stable, reproducible and epidemiologically useful.

Citation

Kumar S, Sharma NC, Singh S, Bhatia R, Singh H. Development of a conjoint phage typing & biotyping schema for Salmonella enterica serovar Senftenberg (S. senftenberg) & the correlation of biotypes with phage types. Indian Journal of Medical Research. 1994 Dec; 100(): 257-61