Development of colorimetric method for the quantification of methyl salicylate in bulk and formulations.
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Date
2014-09
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Abstract
Methyl salicylate is chemically methyl-O-hydroxybenzoate and act as an active ingredient in many topical
analgesic preparations. It posses good counter irritant activity. In this present study, a colorimetric method is
developed for the quantitative estimation of methyl salicylate, both in the bulk form and in the formulations. A
dark bluish – purple colour is formed between methyl salicylate and ferric chloride reagent. The intensity of the
colour is directly related to the concentration of methyl salicylate present. The developed method was simple and
gave accurate and precise results. The developed colour had its maximum value of absorbance at 537 nm when
scanned in the visible region. The absorbance of the coloured system was measured at 537 nm. Beer’s law was
obeyed in the range of 12 to 72 μg/mL. The equation for the straight line was found to be, y = 0.0075 x + 0.002
with correlation coefficient value of 0.996. The developed method was applied to semi – solid formulations for
the quantitative estimation of methyl salicylate. The percentage of recovery was between 99.62 % in the
formulation. The method proved to be a convenient analytical tool for the quantity determination of methyl
salicylate.
Description
Keywords
Methyl salicylate, Ferric chloride, colorimetry
Citation
Abbas Sajin Kattuvilakam, Rengitham Suseem Sundaram. Development of colorimetric method for the quantification of methyl salicylate in bulk and formulations. Journal of Applied Pharmaceutical Science. 2014 Sept; 4(9): 52-55.