Clinical utility of screening for antinuclear antibodies by enzyme immunoassay--a preliminary study.

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dc.contributor.authorDivate, Sen_US
dc.contributor.authorHardikar, Pen_US
dc.contributor.authorBichile, L Sen_US
dc.contributor.authorRajadhyaksha, Aen_US
dc.date.accessioned2004-04-08en_US
dc.date.accessioned2009-05-31T02:33:34Z
dc.date.available2004-04-08en_US
dc.date.available2009-05-31T02:33:34Z
dc.date.issued2004-04-08en_US
dc.description.abstractAIMS OF THE STUDY: To evaluate the advantages and reliability of screening for antinuclear antibodies (ANA) by enzyme immunoassay (ELISA). METHODOLOGY: Sera from 96 patients comprising 51 with systemic lupus erythematosus (SLE), 11 with other systemic rheumatological diseases (SRD) and 34 with various other diseases (non-SRD) were tested using a commercial ELISA kit (ANA-Ease, Genesis Biotechnology, U.K.). These sera consisted of 53 immunofluorescence assay (IF) ANA-positive and 43 IF ANA-negative samples RESULTS: We observed that when compared to the IF for ANA the sensitivity, specificity, predictive values for positives (PPV) and negatives (NPV) of ELISA were 90.7%, 85.7%, 89.1% and 87.8% respectively. Exclusion of borderline ELISA positive by slightly raising the cut-off optical density (OD) increased the specificity and PPV to 93.1%, and 94.1% respectively. Importantly, none of the non-SRD sera were positive when this higher cut-off was used. ELISA was noted to be strongly positive in three IF ANA-negative SLE patients. However there was no correlation between the ELISA ANA semi-quantitative index and the IF ANA titers. CONCLUSIONS: ELISA appears to be suitable as a preliminary screening test for ANA. An appropriate cut-off should be identified to segregate low positive samples that could be false-positives. Nevertheless, IF will need to be performed to estimate the titers, identify patterns of ANA positive samples and confirm results of low positive "gray-zone" samples and ELISA negative sera from patients with a high index of clinical suspicion of SLE.en_US
dc.description.affiliationDepartment of Pathology and Medicine, Seth G. S. Medical College and K.E.M. Hospital, Parel, Mumbai.en_US
dc.identifier.citationDivate S, Hardikar P, Bichile LS, Rajadhyaksha A. Clinical utility of screening for antinuclear antibodies by enzyme immunoassay--a preliminary study. Journal of the Association of Physicians of India. 2004 Apr; 52(): 290-3en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/93146
dc.language.isoengen_US
dc.source.urihttps://www.japi.orgen_US
dc.subject.meshAntibodies, Antinuclear --blooden_US
dc.subject.meshBiological Markers --blooden_US
dc.subject.meshEnzyme-Linked Immunosorbent Assayen_US
dc.subject.meshFalse Negative Reactionsen_US
dc.subject.meshFalse Positive Reactionsen_US
dc.subject.meshHumansen_US
dc.subject.meshLupus Erythematosus, Systemic --diagnosisen_US
dc.subject.meshMass Screening --methodsen_US
dc.subject.meshPredictive Value of Testsen_US
dc.subject.meshSensitivity and Specificityen_US
dc.titleClinical utility of screening for antinuclear antibodies by enzyme immunoassay--a preliminary study.en_US
dc.typeEvaluation Studiesen_US
dc.typeJournal Articleen_US
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