Comparison between the antimicrobial susceptibility of Burkholderia pseudomallei to trimethoprim-sulfamethoxazole by standard disk diffusion method and by minimal inhibitory concentration determination.

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dc.contributor.authorLumbiganon, Pen_US
dc.contributor.authorTattawasatra, Uen_US
dc.contributor.authorChetchotisakd, Pen_US
dc.contributor.authorWongratanacheewin, Sen_US
dc.contributor.authorThinkhamrop, Ben_US
dc.date.accessioned2009-05-27T19:10:45Z
dc.date.available2009-05-27T19:10:45Z
dc.date.issued2000-08-22en_US
dc.descriptionChotmaihet Thangphaet.en_US
dc.description.abstractMelioidosis, an infection caused by Burkholderia pseudomallei, usually occurs in immunocompromised patients and requires prolonged antibiotic therapy. Previously, oral trimethoprim-sulfamethoxazole (TM/SM), an inexpensive and effective drug has been used as a maintenance therapy. The susceptibility of B. pseudomallei to TM/SM by the standard disk diffusion method is very low. However, some patients who were treated with TM/SM as a maintenance therapy despite the in vitro resistance showed good clinical responses. There were no data comparing the susceptibility of B. pseudomallei by the standard disk diffusion method with other quantitative susceptibility tests. The objective of this study was to determine the agreement between the antimicrobial susceptibility of B. pseudomallei to TM/SM by standard disk diffusion and minimal inhibitory concentration determination (MIC). We performed the susceptibility test of 144 strains of B. pseudomallei to TM/SM by both the standard disk diffusion and microbroth dilution MIC. The sensitivity results were 53.5 per cent and 84.0 per cent respectively. The agreement between the 2 tests was very poor (Kappa = 0.14; 95% CI = -0.01 to 0.29). The false resistant rate by the standard disk diffusion test was 67.9 per cent. Further in vitro susceptibility and clinical study are needed to define the interpretive criteria that correlate with clinical response.en_US
dc.description.affiliationDepartment of Pediatrics, Faculty of Public Health, Khon Kaen University, Thailand.en_US
dc.identifier.citationLumbiganon P, Tattawasatra U, Chetchotisakd P, Wongratanacheewin S, Thinkhamrop B. Comparison between the antimicrobial susceptibility of Burkholderia pseudomallei to trimethoprim-sulfamethoxazole by standard disk diffusion method and by minimal inhibitory concentration determination. Journal of the Medical Association of Thailand. 2000 Aug; 83(8): 856-60en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/40622
dc.language.isoengen_US
dc.source.urihttps://www.mat.or.th/journal/all.phpen_US
dc.subject.meshAdministration, Oralen_US
dc.subject.meshAnti-Bacterial Agents --pharmacologyen_US
dc.subject.meshBurkholderia pseudomallei --classificationen_US
dc.subject.meshDrug Resistance, Microbialen_US
dc.subject.meshFalse Positive Reactionsen_US
dc.subject.meshHumansen_US
dc.subject.meshInhibitory Concentration 50en_US
dc.subject.meshMelioidosis --drug therapyen_US
dc.subject.meshMicrobial Sensitivity Tests --methodsen_US
dc.subject.meshReproducibility of Resultsen_US
dc.subject.meshSensitivity and Specificityen_US
dc.subject.meshSerotypingen_US
dc.subject.meshTrimethoprim-Sulfamethoxazole Combination --pharmacologyen_US
dc.titleComparison between the antimicrobial susceptibility of Burkholderia pseudomallei to trimethoprim-sulfamethoxazole by standard disk diffusion method and by minimal inhibitory concentration determination.en_US
dc.typeComparative Studyen_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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