Molecular genotyping of clinically important blood group antigens in patients with thalassaemia

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dc.contributor.authorKulkarni, Sen_US
dc.contributor.authorChoudhary, Ben_US
dc.contributor.authorGogri, Hen_US
dc.contributor.authorPatil, Sen_US
dc.contributor.authorManglani, Men_US
dc.contributor.authorSharma, Ren_US
dc.contributor.authorMadkaikar, Men_US
dc.date.accessioned2020-04-10T01:40:41Z
dc.date.available2020-04-10T01:40:41Z
dc.date.issued2018-12
dc.description.abstractBackground & objectives: In multitransfused thalassaemic patients, haemagglutination fails to phenotype the patient's blood group antigens due to the presence of donor-derived erythrocytes. DNA-based methods can overcome the limitations of haemagglutination and can be used to determine the correct antigen profile of these patients. This will facilitate the procurement of antigen-matched blood for transfusion to multitransfused patients. Thus, the aim of this study was to compare the serological phenotyping of common and clinically important antigens of Rh, Duffy, Kell, Kidd and MNS blood group systems with molecular genotyping amongst multitransfused thalassaemic patients. Methods: Blood samples from 200 patients with thalassaemia and 100 'O' group regular blood donors were tested using standard serological techniques and polymerase chain reaction-based methods for common antigens/alleles (C, c, D, E, e, Fya, Fyb, Jka, Jkb, K, k, M, N, S, s). Results: Genotyping and phenotyping results were discordant in 77 per cent of thalassaemic patients for five pairs of antithetical antigens of Rh, Duffy, Kell and Kidd blood group systems. In the MNS blood group system, 59.1 per cent of patients showed discrepancy. The rate of alloimmunization among thalassaemics was 7.5 per cent. Interpretation & conclusions: Molecular genotyping enabled the determination of the actual antigen profile in multitransfused thalassaemia patients. This would help reduce the problem of alloimmunization in such patients and would also aid in the better management of transfusion therapy.en_US
dc.identifier.affiliationsDepartment of Transfusion Medicine, ICMR-National Institute of Immunohaematology, KEM Hospital Campus, Mumbai, Indiaen_US
dc.identifier.affiliationsArpan Blood Bank, Nashik, Indiaen_US
dc.identifier.affiliationsPediatric Hematology-Oncology & BMT Centre, Lokmanya Tilak Municipal General Hospital, Mumbai, Indiaen_US
dc.identifier.citationKulkarni S, Choudhary B, Gogri H, Patil S, Manglani M, Sharma R, Madkaikar M. Molecular genotyping of clinically important blood group antigens in patients with thalassaemia. Indian Journal of Medical Research. 2018 Dec; 148(6): 713-720en_US
dc.identifier.issn0971-5916
dc.identifier.issn0975-9174
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/195736
dc.languageenen_US
dc.publisherIndian Council of Medical Researchen_US
dc.relation.issuenumber6en_US
dc.relation.volume148en_US
dc.source.urihttps://dx.doi.org/10.4103/ijmr.IJMR_455_17en_US
dc.subjectBlood group genotypingen_US
dc.subjectblood group systemsen_US
dc.subjecthaemagglutinationen_US
dc.subjectmolecular genotypingen_US
dc.subjectpolymerase chain reaction-sequence-specific primeren_US
dc.subjectthalassaemiaen_US
dc.titleMolecular genotyping of clinically important blood group antigens in patients with thalassaemiaen_US
dc.typeJournal Articleen_US
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