Cannabis as homeopathic medicine in extreme dilutions: Thermal analysis for their differentiation and action on a protein

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dc.contributor.authorMondal, Priyaen_US
dc.contributor.authorSukul, Nirmal Chandraen_US
dc.contributor.authorKonar, Athenien_US
dc.contributor.authorSarkar, Tandraen_US
dc.contributor.authorSohel, Md Amiren_US
dc.contributor.authorSengupta, Asmitaen_US
dc.contributor.authorChakraborty, Indranien_US
dc.contributor.authorSukul, Anirbanen_US
dc.date.accessioned2020-01-02T06:23:24Z
dc.date.available2020-01-02T06:23:24Z
dc.date.issued2019-12
dc.description.abstractCannabis indica and C. sativa have been used in homeopathy in extreme dilutions, called potencies, for therapeutic purposes since 1841. The purpose of the present study is to see whether Cannabis dilutions have specific levels of free water molecules which characterize other homeopathic potencies. The second objective is to see whether Cannabis mother tincture (MT) and potencies act on the binding sites of a protein. The three potencies 8, 14 and 32 cH were prepared from Cannabis mother tincture (MT) by successive dilution followed by succussion in 8, 14 and 32 steps, respectively. The 3 potencies of diluent medium 90% EtOH were similarly prepared. Each potency was analysed by differential scanning calorimetry (DSC) to determine the free water level in it. The drug potencies and unpotentised EtOH were tested for their binding reaction with a protein human serum albumin (HSA) by isothermal calorimetry (ITC). MTs and the potencies differ from each other and also from water control and EtOH with respect to free water content as revealed by DSC. MTs, their potencies and EtOH bind to HSA showing difference in thermodynamic parameters in terms of stoichiometry, binding constant, change in enthalpy, entropy and Gibbs free energy. Potencies may initiate their individual effect through binding with a protein thereby leading to subsequent biochemical events inside the cell.en_US
dc.identifier.affiliationsSukul Institute of Homeopathic Research, Santiniketan, West Bengal- 731 235, Indiaen_US
dc.identifier.affiliationsDepartment of Zoology; 3Department of Physics, Visva-Bharati University, Santiniketan, West Bengal- 731 235, Indiaen_US
dc.identifier.affiliationsDepartment of Zoology, Jogamaya Debi College, Kolkata, West Bengal- 700 026, Indiaen_US
dc.identifier.citationMondal Priya, Sukul Nirmal Chandra, Konar Atheni, Sarkar Tandra, Sohel Md Amir, Sengupta Asmita, Chakraborty Indrani, Sukul Anirban. Cannabis as homeopathic medicine in extreme dilutions: Thermal analysis for their differentiation and action on a protein. Indian Journal of Biochemistry and Biophysics. 2019 Dec; 56(6): 506-513en_US
dc.identifier.issn0975-0959
dc.identifier.issn0301-1208
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/190084
dc.languageenen_US
dc.publisherNISCAIR-CSIR, Indiaen_US
dc.relation.issuenumber6en_US
dc.relation.volume56en_US
dc.source.urihttps://nopr.niscair.res.in/handle/123456789/52163en_US
dc.titleCannabis as homeopathic medicine in extreme dilutions: Thermal analysis for their differentiation and action on a proteinen_US
dc.typeJournal Articleen_US
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