Retrospective study on the diagnostic value of IgG ELISA, dot immunoassay and indirect hemagglutination in septicemic melioidosis.

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dc.contributor.authorWongratanacheewi, Sen_US
dc.contributor.authorSermswan, R Wen_US
dc.contributor.authorAnuntagool, Nen_US
dc.contributor.authorSirisinha, Sen_US
dc.date.accessioned2009-05-27T17:27:07Z
dc.date.available2009-05-27T17:27:07Z
dc.date.issued2001-06-09en_US
dc.descriptionPublished by the Allergy and Immunology Society of Thailand.en_US
dc.description.abstractThree serological methods for diagnosis of melioidosis were compared with the culture method currently used as the "gold standard". The diagnostic values of the serological methods were evaluated retrospectively in 306 patients residing in an endemic area. The enzyme-linked immunosorbent assay (ELISA), using affinity purified antigen for detecting specific IgG antibody, showed a slightly higher specificity (86.0%) than the dot immunoassay (DOT) (84.0%) and both were superior to indirect hemagglutination (IHA) (72.0%). The sensitivity of DOT (96.4%) and ELISA (85.7%) were considerably higher than that of IHA (50.0%). The primary benefit of the high negative predictive value of both ELISA (96.4%) and DOT (99.0%) in an area of high prevalence is the ability to rule out most of the non-melioidosis patients.en_US
dc.description.affiliationDepartment of Microbiology, Faculty of Medicine, Khon Kaen University, Thailand.en_US
dc.identifier.citationWongratanacheewi S, Sermswan RW, Anuntagool N, Sirisinha S. Retrospective study on the diagnostic value of IgG ELISA, dot immunoassay and indirect hemagglutination in septicemic melioidosis. Asian Pacific Journal of Allergy and Immunology. 2001 Jun; 19(2): 129-33en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/37222
dc.language.isoengen_US
dc.subject.meshBacteremia --blooden_US
dc.subject.meshBurkholderia pseudomallei --isolation & purificationen_US
dc.subject.meshEnzyme-Linked Immunosorbent Assayen_US
dc.subject.meshFalse Positive Reactionsen_US
dc.subject.meshHemagglutination Testsen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunoblottingen_US
dc.subject.meshImmunoglobulin G --blooden_US
dc.subject.meshMelioidosis --blooden_US
dc.subject.meshPredictive Value of Testsen_US
dc.subject.meshRetrospective Studiesen_US
dc.subject.meshSensitivity and Specificityen_US
dc.subject.meshThailanden_US
dc.titleRetrospective study on the diagnostic value of IgG ELISA, dot immunoassay and indirect hemagglutination in septicemic melioidosis.en_US
dc.typeComparative Studyen_US
dc.typeEvaluation Studiesen_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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