Molecular cloning of phospholipase A2 from a Thai Russell's viper venom gland cDNA library.

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dc.contributor.authorNuchprayoon, Ien_US
dc.contributor.authorSai-Ngam, Aen_US
dc.contributor.authorSuntrarachun, Sen_US
dc.contributor.authorNoiphrom, Jen_US
dc.contributor.authorPakmanee, Nen_US
dc.contributor.authorChanhome, Len_US
dc.contributor.authorNuchprayoon, Sen_US
dc.contributor.authorSitprija, Ven_US
dc.date.accessioned2009-05-27T20:19:36Z
dc.date.available2009-05-27T20:19:36Z
dc.date.issued2001-06-01en_US
dc.descriptionChotmaihet Thangphaet.en_US
dc.description.abstractSnake venom contains several toxins. Russell's viper (D. russellii, RV) is a venomous snake prevalent in northern and central Thailand. RV bites can cause disseminated coagulation, hemolysis, and edema of the bitten limbs. To identify protein components of RV venom, we made a cDNA library from RV venom glands, and randomly sequenced cloned cDNA. We were able to clone a cDNA encoding RV phospholipase A2 (PLA2). PLA2 is an active enzyme found in several species of snake venom worldwide. PLA2 is thought to be toxic to cell membrane, thereby, can cause local cell and tissue damage, as well as systemic effects in snake bite victims. This PLA2 cDNA clone would facilitate in vivo studies of the pathophysiology of RV bite.en_US
dc.description.affiliationDepartment of Pediatrics, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.en_US
dc.identifier.citationNuchprayoon I, Sai-Ngam A, Suntrarachun S, Noiphrom J, Pakmanee N, Chanhome L, Nuchprayoon S, Sitprija V. Molecular cloning of phospholipase A2 from a Thai Russell's viper venom gland cDNA library. Journal of the Medical Association of Thailand. 2001 Jun; 84 Suppl 1(): S99-105en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/42791
dc.language.isoengen_US
dc.source.urihttps://www.mat.or.th/journal/all.phpen_US
dc.subject.meshAmino Acid Sequenceen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCloning, Molecularen_US
dc.subject.meshDNA, Complementary --analysisen_US
dc.subject.meshGene Libraryen_US
dc.subject.meshPhospholipases A --geneticsen_US
dc.subject.meshPhospholipases A2en_US
dc.subject.meshSensitivity and Specificityen_US
dc.subject.meshSnake Bites --enzymologyen_US
dc.subject.meshThailand --epidemiologyen_US
dc.subject.meshViper Venoms --enzymologyen_US
dc.subject.meshViperidaeen_US
dc.titleMolecular cloning of phospholipase A2 from a Thai Russell's viper venom gland cDNA library.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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