Quantitative analysis of fibrillar collagen organization in the immediate proximity of embedded fibroblasts in 3D collagen hydrogels

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dc.contributor.authorKANADE, SHAUNAKen_US
dc.contributor.authorDESAI, MILIEen_US
dc.contributor.authorBHATAVADEKAR, NEELen_US
dc.contributor.authorBALASUBRAMANIAN, NAGARAJen_US
dc.date.accessioned2024-11-30T11:24:43Z
dc.date.available2024-11-30T11:24:43Z
dc.date.issued2024-06
dc.description.abstractFibroblasts embedded in a 3D matrix microenvironment can remodel the matrix to regulate cell adhesion and function. Collagen hydrogels are a useful in vitro system to study cell–matrix interactions in a 3D microenvironment. While major matrix reorganizations are easily recognizable, subtle changes in response to environmental or biochemical cues are challenging to discern in 3D hydrogels. Three-dimensional collagen gels at 1.0 mg/ml vs 1.5 mg/ml were labelled with DQ-collagen and imaged by confocal reflectance microscopy to evaluate these small changes. An image analysis pipeline was developed, hydrogel area and number of cross-sections analysed were optimized, and fibrillar collagen properties (number of branches, number of junctions, and average branch length) were quantified. While no significant changes were seen in fibrillar collagen organization between 1.0 mg/ml and 1.5 mg/ml collagen hydrogels, embedded mouse fibroblasts caused a significant increase in collagen branching and organization. Using the phalloidin-labelled cells, this change was quantitated in immediate proximity of the cell. A distinct increase in branch and junction numbers was observed, significantly altered by small changes in collagen concentration (1.0 mg/ml vs 1.5 mg/ml). Together, this analysis gives a quantitative evaluation of how cells respond to and modify their immediate microenvironment in a 3D collagen hydrogel.en_US
dc.identifier.affiliationsIndian Institute of Science Education and Research (IISER), Pune, Dr. Homi Bhabha Road, Pashan, Pune 411 008, Indiaen_US
dc.identifier.affiliationsIndian Institute of Science Education and Research (IISER), Pune, Dr. Homi Bhabha Road, Pashan, Pune 411 008, Indiaen_US
dc.identifier.affiliationsClarus Dental Specialities, 1st Floor, Serene Bay, Lane 6, Koregaon Park, Pune 411 001, India; Present Address: Department of Periodontology, Tufts University, Boston, USAen_US
dc.identifier.affiliationsIndian Institute of Science Education and Research (IISER), Pune, Dr. Homi Bhabha Road, Pashan, Pune 411 008, Indiaen_US
dc.identifier.citationKANADE SHAUNAK, DESAI MILIE, BHATAVADEKAR NEEL, BALASUBRAMANIAN NAGARAJ. Quantitative analysis of fibrillar collagen organization in the immediate proximity of embedded fibroblasts in 3D collagen hydrogels. Journal of Biosciences. 2024 Jun; 49: 1-11en_US
dc.identifier.issn0250-5991
dc.identifier.issn0973-7138
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/237983
dc.languageenen_US
dc.publisherThe Indian Academy of Sciencesen_US
dc.relation.volume49en_US
dc.source.urihttps://doi.org/10.1007/s12038-024-00449-4en_US
dc.subjectCollagen organizationen_US
dc.subjectextracellular matricesen_US
dc.subjectfibroblasten_US
dc.subjectreflectance imagingen_US
dc.subject3D hydrogelen_US
dc.titleQuantitative analysis of fibrillar collagen organization in the immediate proximity of embedded fibroblasts in 3D collagen hydrogelsen_US
dc.typeJournal Articleen_US
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