Minor Sesquiterpenoid and Steroid Constitutes from Azadirachta indica A. Juss and Their Cytotoxic Activity

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dc.contributor.authorDong, Huaen_US
dc.contributor.authorXiaofeng Luen_US
dc.contributor.authorZi, Jiachenen_US
dc.contributor.authorFan, Xiaonaen_US
dc.date.accessioned2020-11-18T10:29:11Z
dc.date.available2020-11-18T10:29:11Z
dc.date.issued2019-05
dc.description.abstractBackground Neem (Azadirachta indica A. Juss) is a plant belonging to the meliaceae family. It was used widely asherbal medicine in ancient India and Burma. It possesses a variety of chemical constitutes. Recent studies showedthat some of major constitutes have remarkable anticancer activities. However, the minor constitutes in neem arestill studied insufficiently.Aim The aim of the study is to identify the minor constitutes in neem, and investigate their cytotoxic activity.Material & methods The dry seeds of neem were extracted with 95% ethanol. The ethyl acetate fraction of theextract was systematically separated by silica gel, Sephadex LH-20, High Performance Liquid Chromatography(HPLC) and other chromatographic techniques. The structures of the resulting isolates were elucidated byspectroscopic methods, such as mass spectrum (MS), nuclear magnetic resonance spectrum (NMR), etc.Results A sesquiterpenoid, 11,13-dihydroqinghaosu V (1), together with two known steroids, (24R)-ergosta5,7,22E-trien-3β-ol (2) and 5α,8α-epidioxiergosta-6,22-dien-3β-ol (3), was isolated from the dry seeds of neem. 1is a compound which is isolated from nature for the first time. In vitro cytotoxic bioassays showed that compound1 selectively inhibited the growth of Hela cell lines, with an IC50 value of 37.26 ±6.02 µM.Conclusion The study indicated that besides some major constituents of neem possessing anticancer activity, itsminor metabolites may also inhibit growth of cancer cellsen_US
dc.identifier.affiliationsCollege of Pharmacy, Jinan University, Guangzhou 510632, Chinaen_US
dc.identifier.affiliationsDispensary of Traditional Chinese Medicine, Hainan Provincial Hospital of Traditional Chinese Medicine, Haikou 570203, People’s Republic of Chinaen_US
dc.identifier.citationDong Hua, Xiaofeng Lu, Zi Jiachen, Fan Xiaona. Minor Sesquiterpenoid and Steroid Constitutes from Azadirachta indica A. Juss and Their Cytotoxic Activity. Journal of Pharmaceutical and Biomedical Sciences. 2019 May; 9(5): 57-61en_US
dc.identifier.issn2230-7885
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/215720
dc.languageenen_US
dc.publisherLawarence Press Pvt. Ltd.en_US
dc.relation.issuenumber5en_US
dc.relation.volume9en_US
dc.source.urihttps://lawarencepress.com/ojs/index.php/JPBMS/article/view/562/pdf_280en_US
dc.subjectAzadirachta indicaen_US
dc.subjectsesquiterpenoiden_US
dc.subjectsteroiden_US
dc.subjectcytotoxic activityen_US
dc.titleMinor Sesquiterpenoid and Steroid Constitutes from Azadirachta indica A. Juss and Their Cytotoxic Activityen_US
dc.typeJournal Articleen_US
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