Coordinated secretion of alkaline phosphatase into serum and intestine in fat-fed rats.
dc.contributor.author | Grewal, R | en_US |
dc.contributor.author | Mahmood, Akhtar | en_US |
dc.date.accessioned | 2004-09-16 | en_US |
dc.date.accessioned | 2009-05-29T02:29:45Z | |
dc.date.available | 2004-09-16 | en_US |
dc.date.available | 2009-05-29T02:29:45Z | |
dc.date.issued | 2004-09-16 | en_US |
dc.description.abstract | BACKGROUND: Fat feeding increases the activity of intestinal alkaline phosphatase in the serum. The mechanism underlying this increase is unknown. Surfactant-like particles (SLP) secreted by enterocytes have been implicated in this phenomenon. OBJECTIVE: To study the effect of feeding fish oil and protein synthesis inhibitors on alkaline phosphatase activity in serum and in different intestinal fractions. METHODS: Male albino rats were fed 2 mL of fish oil and were injected cycloheximide or actinomycin D. Alkaline phosphatase activity was determined in the serum and intestinal fractions (SLP, mucosa, muscularis). RESULT: Feeding fish oil significantly elevated alkaline phosphatase activity in serum (p< 0.001) and intestinal mucosa (p< 0.01). Administration of cycloheximide or actinomycin D significantly reduced alkaline phosphatase activity in serum (p< 0.01) and in intestinal mucosa (p< 0.05). BCIP staining of brush border alkaline phosphatase activity in acrylamide gels yielded similar results. CONCLUSIONS: These findings suggest a co-ordination between alkaline phosphatase synthesis and its assembly into lipoprotein vesicles, such as SLP, secreted by enterocytes in response to fat feeding. | en_US |
dc.description.affiliation | Department of Biochemistry, Panjab University, Sector 14, Chandigarh-160 014, India. | en_US |
dc.identifier.citation | Grewal R, Mahmood A. Coordinated secretion of alkaline phosphatase into serum and intestine in fat-fed rats. Indian Journal of Gastroenterology. 2004 Sep-Oct; 23(5): 175-7 | en_US |
dc.identifier.uri | https://imsear.searo.who.int/handle/123456789/64211 | |
dc.language.iso | eng | en_US |
dc.source.uri | https://www.indianjgastro.com | en_US |
dc.subject.mesh | Alkaline Phosphatase --blood | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Dietary Fats --metabolism | en_US |
dc.subject.mesh | Disease Models, Animal | en_US |
dc.subject.mesh | Gastrointestinal Motility --physiology | en_US |
dc.subject.mesh | Intestinal Mucosa --enzymology | en_US |
dc.subject.mesh | Intestine, Small --enzymology | en_US |
dc.subject.mesh | Male | en_US |
dc.subject.mesh | Metabolic Clearance Rate | en_US |
dc.subject.mesh | Probability | en_US |
dc.subject.mesh | Rats | en_US |
dc.subject.mesh | Rats, Inbred Strains | en_US |
dc.subject.mesh | Reference Values | en_US |
dc.subject.mesh | Sensitivity and Specificity | en_US |
dc.title | Coordinated secretion of alkaline phosphatase into serum and intestine in fat-fed rats. | en_US |
dc.type | Comparative Study | en_US |
dc.type | Journal Article | en_US |
dc.type | Research Support, Non-U.S. Gov't | en_US |
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