Coordinated secretion of alkaline phosphatase into serum and intestine in fat-fed rats.

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dc.contributor.authorGrewal, Ren_US
dc.contributor.authorMahmood, Akhtaren_US
dc.date.accessioned2004-09-16en_US
dc.date.accessioned2009-05-29T02:29:45Z
dc.date.available2004-09-16en_US
dc.date.available2009-05-29T02:29:45Z
dc.date.issued2004-09-16en_US
dc.description.abstractBACKGROUND: Fat feeding increases the activity of intestinal alkaline phosphatase in the serum. The mechanism underlying this increase is unknown. Surfactant-like particles (SLP) secreted by enterocytes have been implicated in this phenomenon. OBJECTIVE: To study the effect of feeding fish oil and protein synthesis inhibitors on alkaline phosphatase activity in serum and in different intestinal fractions. METHODS: Male albino rats were fed 2 mL of fish oil and were injected cycloheximide or actinomycin D. Alkaline phosphatase activity was determined in the serum and intestinal fractions (SLP, mucosa, muscularis). RESULT: Feeding fish oil significantly elevated alkaline phosphatase activity in serum (p< 0.001) and intestinal mucosa (p< 0.01). Administration of cycloheximide or actinomycin D significantly reduced alkaline phosphatase activity in serum (p< 0.01) and in intestinal mucosa (p< 0.05). BCIP staining of brush border alkaline phosphatase activity in acrylamide gels yielded similar results. CONCLUSIONS: These findings suggest a co-ordination between alkaline phosphatase synthesis and its assembly into lipoprotein vesicles, such as SLP, secreted by enterocytes in response to fat feeding.en_US
dc.description.affiliationDepartment of Biochemistry, Panjab University, Sector 14, Chandigarh-160 014, India.en_US
dc.identifier.citationGrewal R, Mahmood A. Coordinated secretion of alkaline phosphatase into serum and intestine in fat-fed rats. Indian Journal of Gastroenterology. 2004 Sep-Oct; 23(5): 175-7en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/64211
dc.language.isoengen_US
dc.source.urihttps://www.indianjgastro.comen_US
dc.subject.meshAlkaline Phosphatase --blooden_US
dc.subject.meshAnimalsen_US
dc.subject.meshDietary Fats --metabolismen_US
dc.subject.meshDisease Models, Animalen_US
dc.subject.meshGastrointestinal Motility --physiologyen_US
dc.subject.meshIntestinal Mucosa --enzymologyen_US
dc.subject.meshIntestine, Small --enzymologyen_US
dc.subject.meshMaleen_US
dc.subject.meshMetabolic Clearance Rateen_US
dc.subject.meshProbabilityen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Inbred Strainsen_US
dc.subject.meshReference Valuesen_US
dc.subject.meshSensitivity and Specificityen_US
dc.titleCoordinated secretion of alkaline phosphatase into serum and intestine in fat-fed rats.en_US
dc.typeComparative Studyen_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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