Chromotropic character of bacterial acidic polysaccharides: Part II--Induction of metachromasy in cationic dye pinacyanol chloride by Klebsiella K10 capsular polysaccharide.

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dc.contributor.authorChakrabarti, Aen_US
dc.contributor.authorNath, R Ken_US
dc.contributor.authorChakraborty, A Ken_US
dc.date.accessioned1989-04-01en_US
dc.date.accessioned2009-05-27T09:06:18Z
dc.date.available1989-04-01en_US
dc.date.available2009-05-27T09:06:18Z
dc.date.issued1989-04-01en_US
dc.description.abstractThe acidic capsular polysaccharide isolated from Klebsiella K10 exhibited chromotropic character with respect to induction of metachromasy in the cationic dye pinacyanol chloride (1-ethyl-2-[3-(1-ethyl-2(1H)-quinolylidene)propenyl]quinolinium chloride). Klebsiella K10 polymer consists of hexasaccharide repeating units containing one residue of glucuronic acid along with other neutral sugars in each repeating unit. It induces a metachromatic blue shift in the visible absorption spectrum of the dye from 600 nm to 500 nm. The spectral changes have been studied during interaction of the dye cations with the polyanions at different polymer/dye molar ratios. The polyanion-dye compounds are formed with polymer/dye stoichiometry of 1:1, indicating formation of stacking conformation. The complete reversal of polymer-induced metachromasy has also been observed by the addition of ethanol and urea.en_US
dc.identifier.citationChakrabarti A, Nath RK, Chakraborty AK. Chromotropic character of bacterial acidic polysaccharides: Part II--Induction of metachromasy in cationic dye pinacyanol chloride by Klebsiella K10 capsular polysaccharide. Indian Journal of Biochemistry & Biophysics. 1989 Apr; 26(2): 74-9en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/26462
dc.language.isoengen_US
dc.source.urihttps://https://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijbb/ijbb0.aspen_US
dc.subject.meshCarbocyanines --metabolismen_US
dc.subject.meshCationsen_US
dc.subject.meshColoring Agentsen_US
dc.subject.meshDrug Interactionsen_US
dc.subject.meshHydrogen-Ion Concentrationen_US
dc.subject.meshKlebsiella --analysisen_US
dc.subject.meshPolysaccharides, Bacterial --pharmacologyen_US
dc.subject.meshQuinolines --metabolismen_US
dc.titleChromotropic character of bacterial acidic polysaccharides: Part II--Induction of metachromasy in cationic dye pinacyanol chloride by Klebsiella K10 capsular polysaccharide.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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