MiR-212-3p inhibits cell proliferation and promotes apoptosis by targeting nuclear factor IA in bladder cancer

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dc.contributor.authorXiaoming, Wuen_US
dc.contributor.authorHao, Chenen_US
dc.contributor.authorGaoyue, Zhangen_US
dc.contributor.authorJianhui, Wuen_US
dc.contributor.authorWei, Zhuen_US
dc.contributor.authorYanqin, Guen_US
dc.contributor.authorYi, Heen_US
dc.date.accessioned2020-11-18T10:14:30Z
dc.date.available2020-11-18T10:14:30Z
dc.date.issued2019-09
dc.description.abstractAccumulating evidence suggest that microRNAs play crucial roles in the development and progression of bladder cancer(BC). Here, we found that miR-212-3p was significantly down-regulated and negatively correlated with nuclear factor IA(NFIA) in human BC tissues. Bioinformatics analysis predicted that NFIA was a target gene of miR-212-3p. Then BC celllines, T24 and J82 cells were transfected with miR-212-3p mimics or siNFIA to obtain miR-212-3p overexpression or NFIAknockdown cell lines, respectively. Quantitative real-time PCR was used to determine the expression of miR-212-3p andNFIA. Western blot analysis was utilized to detect NFIA expression. MTT assay showed either miR-212-3 overexpression orNFIA knockdown significantly inhibited the BC cell proliferation. Double staining with Annexin V-APC and 7-AAD showedthe total number of apoptotic BC cells were remarkably increased after miR-212-3p overexpression or NFIA knockdown.Collectively, our results indicated that miR-212-3p targeting NFIA might serve as a promising target for BC.en_US
dc.identifier.affiliationsDepartment of Urology, the First Hospital of Jiaxing, Jiaxing, Zhejiang Province, People’s Republic of Chinaen_US
dc.identifier.citationXiaoming Wu, Hao Chen, Gaoyue Zhang, Jianhui Wu, Wei Zhu, Yanqin Gu, Yi He. MiR-212-3p inhibits cell proliferation and promotes apoptosis by targeting nuclear factor IA in bladder cancer. Journal of Biosciences. 2019 Sep; 44(4): 1-8en_US
dc.identifier.issn0250-5991
dc.identifier.issn0973-7138
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/214423
dc.languageenen_US
dc.publisherIndian Academy of Sciencesen_US
dc.relation.issuenumber4en_US
dc.relation.volume44en_US
dc.source.urihttps://dx.doi.org//10.1007/s12038-019-9903-5en_US
dc.subjectApoptosisen_US
dc.subjectbladder canceren_US
dc.subjectmiR-212-3pen_US
dc.subjectNFIAen_US
dc.subjectproliferationen_US
dc.titleMiR-212-3p inhibits cell proliferation and promotes apoptosis by targeting nuclear factor IA in bladder canceren_US
dc.typeJournal Articleen_US
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