Utility of polyclonal antibodies binding to specific epitopes of Burkholderia pseudomallei

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dc.contributor.authorDarawan Rinchaien_US
dc.contributor.authorAroonlug Lulitanonden_US
dc.contributor.authorGanjana Lertmemongkolchaien_US
dc.date.accessioned2011-02-20T04:32:54Z
dc.date.available2011-02-20T04:32:54Z
dc.date.created2009-12-25en_US
dc.date.issued2009-12-25en_US
dc.description.abstractBurkholderia pseudomallei (B. pseudomallei) is a major causative agent of melioidosis in endemic areas such as Southeast Asia and Northern Australia. Rapid diagnosis is required for appropriate treatments in septicemic melioidosis. In this study, we aimed to evaluate the specific polyclonal antibody (pAb) to B. pseudomallei for potentially use in the development of diagnostic assays and pathogenesis studies. In determination the cross reaction of the pAb to other bacteria by indirect ELISA, the pAb to B. pseudomallei has no cross-reactivity with other bacteria. In contrast, the pAb reacted with 24 clinical isolates of B. pseudomallei-extracted antigens. By ELISA, the pAb to B. pseudomallei could be used to detect secreted antigens in 3 hour culture supernatants of 1.5 x 104 cfu/ml B. pseudomallei. In addition, opsonization of the bacteria with the pAbs could enhance bacterial internalization by U937-derived macrophages when compared with bacterial culture alone. The mechanism of these observations, however, needs to be further investigated. In conclusion, we suggest that the pAbs to B. pseudomallei can be potentially used for development of diagnosis method and pathogenesis study.en_US
dc.identifier.citationJournal of Medical Technology and Physical Therapy; Volume 21 Number 1, January - April 2009; 14 - 22en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/130816
dc.language.isoen_USen_US
dc.publisherKhon Kaen Universityen_US
dc.rightsKhon Kaen Universityen_US
dc.source.urihttps://thailand.digitaljournals.org/index.php/JMTPT/issue/archiveen_US
dc.source.urihttps://thailand.digitaljournals.org/index.php/JMTPT/article/view/276en_US
dc.titleUtility of polyclonal antibodies binding to specific epitopes of Burkholderia pseudomalleien_US
dc.typeORIGINAL ARTICLEen_US
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