Detection of Helicobactor pylori by polymerase chain reaction: A comparison in sample preparation.

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dc.contributor.authorMurugesan, R
dc.contributor.authorBhattacharjee, P
dc.contributor.authorKumar, P R Vivek
dc.contributor.authorMohankumar, Mary N
dc.contributor.authorNagarajan, Mangala
dc.contributor.authorJeevanram, R K
dc.date.accessioned2012-11-26T08:12:46Z
dc.date.available2012-11-26T08:12:46Z
dc.date.issued2005-01
dc.description.abstractGastric biopsy samples obtained from 14 patients with upper abdominal pain, clinically diagnosed as acid peptic disease, were analysed for the presence of Helicobacter pylori (H. pylori) by Polymerase Chain Reaction (PCR) using partially (template A) and completely purified DNA (template B). Antigen specific primer was used to analyse the sample by PCR method. The presence of H. pylori in the samples was confirmed by running a positive control. The presence of H. pylori was also detected by urease method using standard protocol. Among the 14 samples studied, 8 showed the presence of H. pylori with both templates A and B. Among these 8 samples only 3 showed positive for the presence of H. pylori with urease method. The present work discusses the results obtained in the detection of H. pylori in template A and B by PCR method.en_US
dc.identifier.citationMurugesan R, Bhattacharjee P, Kumar P R Vivek, Mohankumar Mary N, Nagarajan Mangala, Jeevanram R K. Detection of Helicobactor pylori by polymerase chain reaction: A comparison in sample preparation. Indian Journal of Human Genetics. 2005 Jan; 11(1): 35-38.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/143326
dc.language.isoenen_US
dc.source.urihttps://www.ijhg.com/article.asp?issn=0971-6866;year=2005;volume=11;issue=1;spage=35;epage=38;aulast=Murugesanen_US
dc.subjectH. pylorien_US
dc.subjectGastritisen_US
dc.subjectDuodenal ulceren_US
dc.subjectPolymerase Chain Reactionen_US
dc.subjectAntigen specific primeren_US
dc.titleDetection of Helicobactor pylori by polymerase chain reaction: A comparison in sample preparation.en_US
dc.typeArticleen_US
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