Isoenzyme patterns of mycobacteria. I. Factors influencing LDH isoenzymes of mycobacteria.

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dc.contributor.authorSharma, V Den_US
dc.contributor.authorKatoch, V Men_US
dc.contributor.authorDatta, A Ken_US
dc.contributor.authorShivannavar, C Ten_US
dc.contributor.authorKannan, K Ben_US
dc.contributor.authorBharadwaj, V Pen_US
dc.date.accessioned1985-01-01en_US
dc.date.accessioned2009-05-28T09:45:10Z
dc.date.available1985-01-01en_US
dc.date.available2009-05-28T09:45:10Z
dc.date.issued1985-01-01en_US
dc.description.abstractCell free extracts of a fast growing mycobacterium (M. phlei) and a slow growing mycobacterium (M. tuberculosis H37Ra) were analysed for lactate dehydrogenase (LDH) isoenzymes under different experimental conditions. It was observed that growth of M. phlei when taken from Lowenstein Jensen (LJ) as well as Sauton's medium showed identical band but for (M. tuberculosis H37Ra the number of bands observed were less when grown on LJ-medium. There was no difference in LDH isoenzyme patterns when the mycobacteria were incubated at 30 degrees C and 37 degrees C and under different pH conditions (6.2-8.2). Actively growing cultures of both the species showed distinct LDH isoenzyme patterns whereas the activity and bands became indistinct in old cultures. The LDH bands from lyophilized growth studied resembled to those of fresh growth. The treatment of growth with 1M NaOH for one hour resulted in marked diminution of LDH activity. Sonication with wet growth weight of 0.5 gm per ml of distilled water was found to give clearer bands as compared to phosphate buffer. No loss of LDH isoenzymes activity was noticed after storing the extracts at -80 degrees C for one month, treating to 58 degrees C for one hour or freezing and thawing for 2 times whereas these isoenzymes were quite unstable at other storage temperatures. Increasing the staining time was found helpful in getting clearer bands when activity was low. It is concluded that the factors studied have important bearing on LDH isoenzyme patterns of mycobacteria and must be kept in mind while studying the LDH zymograms for any taxonomic identification of mycobacteria or for studying the metabolic role. These are important both for sensitivity and reproducibility of LDH zymograms.en_US
dc.identifier.citationSharma VD, Katoch VM, Datta AK, Shivannavar CT, Kannan KB, Bharadwaj VP. Isoenzyme patterns of mycobacteria. I. Factors influencing LDH isoenzymes of mycobacteria. Indian Journal of Leprosy. 1985 Jan-Mar; 57(1): 97-106en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/54352
dc.language.isoengen_US
dc.subject.meshCulture Mediaen_US
dc.subject.meshFreezingen_US
dc.subject.meshHydrogen-Ion Concentrationen_US
dc.subject.meshIsoenzymesen_US
dc.subject.meshL-Lactate Dehydrogenase --analysisen_US
dc.subject.meshMycobacterium --enzymologyen_US
dc.subject.meshMycobacterium phlei --enzymologyen_US
dc.subject.meshMycobacterium tuberculosis --enzymologyen_US
dc.subject.meshTemperatureen_US
dc.titleIsoenzyme patterns of mycobacteria. I. Factors influencing LDH isoenzymes of mycobacteria.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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