The temperature--dependent expression of GST of Schistosoma japonicum (Philippine strain).

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dc.contributor.authorCai, Z Hen_US
dc.contributor.authorSong, G Cen_US
dc.contributor.authorXu, Y Xen_US
dc.contributor.authorLiu, S Xen_US
dc.date.accessioned2009-05-27T16:44:25Z
dc.date.available2009-05-27T16:44:25Z
dc.date.issued1993-03-01en_US
dc.descriptionThe Southeast Asian Journal of Tropical Medicine and Public Health.en_US
dc.description.abstractObtained from pSj5, the cDNA gene encoding GST antigen of Schistosoma japonicum (Philippine strain) was ligated with efficient temperature-dependent PBV220 vector which was constructed in CAPM, and then introduced into host bacterium-DH5 alpha (E. coli) by transformation. Transformants were selected by ampicillin and recombinant clones were identified by restriction mapping. The result showed that recombinant clone 43 was the one carrying recombinant plasmid PBV 220 with the correct insertion of the gene fragment. The GST expression ability of clone 43 was investigated by GST enzymic activity assay and SDS-PAGE. A relatively high level of GST enzymic activity was expressed by this clone under the temperature-dependent condition, that is, cultured at 30 degrees C and expressed at 42 degrees C. A more strongly stained 26 kDa protein band was identified by SDS-PAGE. The result indicated that GST of S. japonicum (Philippine strain) could be expressed not only by IPTG induction, but also by the temperature-dependent method.en_US
dc.description.affiliationDepartment of Immunology, Chinese Academy of Preventive Medicine, WHO Collaborating Center of Malaria, Schistosomiasis and Filaliaris, Shanghai.en_US
dc.identifier.citationCai ZH, Song GC, Xu YX, Liu SX. The temperature--dependent expression of GST of Schistosoma japonicum (Philippine strain). The Southeast Asian Journal of Tropical Medicine and Public Health. 1993 Mar; 24(1): 57-60en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/35902
dc.language.isoengen_US
dc.source.urihttps://www.tm.mahidol.ac.th/seameo/publication.htmen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntigens, Helminth --biosynthesisen_US
dc.subject.meshGenetic Engineeringen_US
dc.subject.meshSchistosoma japonicum --immunologyen_US
dc.subject.meshTemperatureen_US
dc.titleThe temperature--dependent expression of GST of Schistosoma japonicum (Philippine strain).en_US
dc.typeJournal Articleen_US
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