Ellagic acid & gallic acid from Lagerstroemia speciosa L. inhibit HIV-1 infection through inhibition of HIV-1 protease & reverse transcriptase activity.

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dc.contributor.authorNutan
dc.contributor.authorModi, Manoj
dc.contributor.authorGoel, Tanvi
dc.contributor.authorDas, Tiyasa
dc.contributor.authorMalik, Shweta
dc.contributor.authorSuri, Samiksha
dc.contributor.authorRawat, Ajay Kumar Singh
dc.contributor.authorSrivastava, Sharad Kumar
dc.contributor.authorTuli, Rakesh
dc.contributor.authorMalhotra, Swadesh
dc.contributor.authorGupta, Satish Kumar
dc.date.accessioned2013-08-06T10:52:56Z
dc.date.available2013-08-06T10:52:56Z
dc.date.issued2013-03
dc.description.abstractBackground & objectives: Banaba (Lagerstroemia speciosa L.) extracts have been used as traditional medicines and are effective in controlling diabetes and obesity. The aim of this study was to evaluate the anti-HIV property of the extracts prepared from the leaves and stems of banaba, and further purification and characterization of the active components. Methods: Aqueous and 50 per cent ethanolic extracts were prepared from leaves and stems of banaba and were evaluated for cytotoxicity and anti-HIV activity using in vitro reporter gene based assays. Further, three compounds were isolated from the 50 per cent ethanolic extract of banaba leaves using silica gel column chromatography and characterization done by HPLC, NMR and MS analysis. To delineate the mode of action of the active compounds, reverse transcriptase assay and protease assay were performed using commercially available kits. Results: All the extracts showed a dose dependent inhibition of HIV-1-infection in TZM-bl and CEM-GFP cell lines with a maximum from the 50 per cent ethanolic extract from leaves (IC50 = 1 to 25 μg/ml). This observation was confirmed by the virus load (p24) estimation in infected CEM-GFP cells when treated with the extracts. Gallic acid showed an inhibition in reverse transcriptase whereas ellagic acid inhibited the HIV-1 protease activity. Interpretation & conclusions: The present study shows a novel anti-HIV activity of banaba. The active components responsible for anti-HIV activity were gallic acid and ellagic acid, through inhibition of reverse transcriptase and HIV protease, respectively and hence could be regarded as promising candidates for the development of topical anti-HIV-1 agents.en_US
dc.identifier.citationNutan, Modi Manoj, Goel Tanvi, Das Tiyasa, Malik Shweta, Suri Samiksha, Rawat Ajay Kumar Singh, Srivastava Sharad Kumar, Tuli Rakesh, Malhotra Swadesh, Gupta Satish Kumar. Ellagic acid & gallic acid from Lagerstroemia speciosa L. inhibit HIV-1 infection through inhibition of HIV-1 protease & reverse transcriptase activity. Indian Journal of Medical Research. 2013 Mar; 137(3): 540-548.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/147754
dc.language.isoenen_US
dc.source.urihttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3705663/?report=classicen_US
dc.subjectAnti-HIV-1 activityen_US
dc.subjectellagic aciden_US
dc.subjectgallic aciden_US
dc.subjectLagerstroemia speciosa L.en_US
dc.subjectproteaseen_US
dc.subjectreverse transcriptaseen_US
dc.subjectrutinen_US
dc.titleEllagic acid & gallic acid from Lagerstroemia speciosa L. inhibit HIV-1 infection through inhibition of HIV-1 protease & reverse transcriptase activity.en_US
dc.typeArticleen_US
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