A Polypeptide Specifically Binds to RAGE can Alleviate the Destroy of Blood Brain-Barrier caused by Amyloid beta

Simple item page
dc.contributor.authorHuang, Yi-yunen_US
dc.contributor.authorZhu, Yu-jieen_US
dc.contributor.authorWang, Shanen_US
dc.contributor.authorZheng, Qingen_US
dc.date.accessioned2020-11-18T10:29:11Z
dc.date.available2020-11-18T10:29:11Z
dc.date.issued2020-04
dc.description.abstractBackground The interaction of the receptor for advanced glycation end product (RAGE) on blood-brain-barrier(BBB) with amyloid β (Aβ) plays an important role in the occurrence and development of AD. RP1 is a RAGEspecific binding peptide, which was discovered in our previous experiments, and it has been proved to beeffective on AD cell model, however, its effects on BBB tight junctions (TJs) and on Aβ transport into the brain isunclear.Methods Immunofluorescence experiment was used to identify whether RP1 bound with RAGE specifically.BEnd3-immortalized mouse brain microvascular endothelial cells were used to construct a BBB model. TEER andFD40 tests were used to confirm the stability of the BBB model, and the colocalization of the RP1 and RAGE onthe surface bEnd3 cells was observed with confocal microscopy.Results We confirmed that RP1 can bind to RAGE specifically in vitro. Functional analyses indicated that RP1 caneffectively alleviate the destroy of TJs of BBB and the decrease of permeability of BBB caused by Aβ. Furthermore,RP1 can competitively inhibit the interaction of Aβ with the RAGE in vitro, and effectively inhibit Aβ transport intothe brain.Conclusion RP1 can inhibit BBB damage induced by Aβ and block RAGE-Aβ interaction effectively, and RP1 canbe a candidate of RAGE inhibitors contributing to AD treatmenten_US
dc.identifier.affiliationsDepartment of Microbial and Biochemical Pharmacy, College of Pharmacy, Jinan University, Guangzhou, Chinaen_US
dc.identifier.citationHuang Yi-yun, Zhu Yu-jie, Wang Shan, Zheng Qing. A Polypeptide Specifically Binds to RAGE can Alleviate the Destroy of Blood Brain-Barrier caused by Amyloid beta. Journal of Pharmaceutical and Biomedical Sciences. 2020 Apr; 10(4): 73-80en_US
dc.identifier.issn2230-7885
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/215716
dc.languageenen_US
dc.publisherLawarence Press Pvt. Ltd.en_US
dc.relation.issuenumber4en_US
dc.relation.volume10en_US
dc.source.urihttps://lawarencepress.com/ojs/index.php/JPBMS/article/view/609/pdf_292en_US
dc.subjectAlzheimer’s diseaseen_US
dc.subjectthe receptor for advanced glycation end product (RAGE)en_US
dc.subjectblood-brain-barrier (BBB)en_US
dc.subjectbeta-amyloid (Aβ)en_US
dc.subjectantagonist peptideen_US
dc.titleA Polypeptide Specifically Binds to RAGE can Alleviate the Destroy of Blood Brain-Barrier caused by Amyloid betaen_US
dc.typeJournal Articleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
jpbms2020v10n4p73.pdf
Size:
1.63 MB
Format:
Adobe Portable Document Format
Download
Collections
Journal of Pharmaceutical and Biomedical Sciences