Comparative analysis of naturally occurring L-amino acid osmolytes and their D-isomers on protection of Escherichia coli against environmental stresses.

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dc.contributor.authorShahjee, Hanief Mden_US
dc.contributor.authorBanerjee, Kakolien_US
dc.contributor.authorAhmad, Faizanen_US
dc.date.accessioned2002-09-17en_US
dc.date.accessioned2009-06-01T14:08:59Z
dc.date.available2002-09-17en_US
dc.date.available2009-06-01T14:08:59Z
dc.date.issued2002-09-17en_US
dc.description.abstractAdaptation to high salinity and low or high temperature is essential for bacteria to survive. Accumulation of exogenous osmolytes is one of the ways that helps bacteria to survive under such extracellular stress. We have analysed the capability of various L-amino acids and their D-isomers to act as osmolytes and thus enable Escherichia coli cells to survive under various stress conditions. E. coli cells were grown in the presence or absence of L- and D-proline, alanine, serine and lysine under salt, heat and cold stresses. Of the various amino acids tested, L-proline, closely followed by L-serine turned out to be highly protective against environmental stresses. L-proline provided excellent protection (95%) against salt stress, followed by cold (60%) and heat (40%) stresses. D-amino acids on the other hand, proved to be highly inhibitory under stress conditions. Thus L-amino acids were found to be growth protectants under stress while their D-isomers were inhibitory during stress as well as normal conditions.en_US
dc.description.affiliationDepartment of Biosciences, Jamia Millia Islamia, Jamia Nagar, New Delhi 110 025, India.en_US
dc.identifier.citationShahjee HM, Banerjee K, Ahmad F. Comparative analysis of naturally occurring L-amino acid osmolytes and their D-isomers on protection of Escherichia coli against environmental stresses. Journal of Biosciences. 2002 Sep; 27(5): 515-20en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/110622
dc.language.isoengen_US
dc.source.urihttps://www.ias.ac.in/jbiosci/index.htmlen_US
dc.subject.meshAmino Acids --chemistryen_US
dc.subject.meshCold Temperatureen_US
dc.subject.meshEscherichia coli --drug effectsen_US
dc.subject.meshHot Temperatureen_US
dc.subject.meshSodium Chlorideen_US
dc.subject.meshStereoisomerismen_US
dc.titleComparative analysis of naturally occurring L-amino acid osmolytes and their D-isomers on protection of Escherichia coli against environmental stresses.en_US
dc.typeComparative Studyen_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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