Correlation of uric acid levels and purine metabolism enzyme activities in plasma and liver tissues of diabetic rats

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dc.contributor.authorGowda, Yogaraje CVen_US
dc.contributor.authorSenthilkumar, Sen_US
dc.contributor.authorKashinath, RTen_US
dc.date.accessioned2020-01-02T06:23:24Z
dc.date.available2020-01-02T06:23:24Z
dc.date.issued2019-12
dc.description.abstractThe uric acid alteration in human beings causes major health problem due to its pivotal role in the etiology of many systemic diseases. The purine metabolism enzyme activities have a significant role in the process of elevated uric acid in diabetes mellitus. Hence, a study has been undertaken to understand the alteration of these enzyme activities in diabetic condition with an attempt to establish the possible cause for uric acid elevation. Alloxan was administered (150 mg/kg; i.p.) to induce diabetes in rats. Thirty days after alloxan induced diabetes, the enzyme activities were assessed in both plasma and liver tissues. The enzyme activities such as 5'-nucleotidase (5'-NT), adenosine deaminase (ADA), xanthine oxidase (XO) in the plasma and liver tissues were assayed by spectrometric technique and uric acid levels were also measured by Caraway procedure. A significant (P< 0.001) increase in 5'-NT, ADA and XO enzyme activities in plasma and liver tissue with a concomitant increase (P< 0.001) in uric acid levels was observed in diabetic group. The uric acid and the activities of enzymes did not change significantly in control group. From the present study, it can be concluded that an increase in uric acid levels noticed in diabetic condition may be due to increased catabolism of purines as evidenced by increased activities of 5'-NT, ADA and XO enzymes.en_US
dc.identifier.affiliationsDepartment of Research and Development, Saveetha Institute of Medical and Technical Sciences, Thandalam, Chennai- 602 105, Tamil Nadu, Indiaen_US
dc.identifier.affiliationsDepartment of Biochemistry, Bangalore Medical College & Research Institute, Bengaluru- 560 002, Karnataka, Indiaen_US
dc.identifier.affiliationsDepartment of Biochemistry, Department of Research and Development, Subbaiah Institute of Medical Sciences, Purle, Shivamogga- 577 222, Karnataka, Indiaen_US
dc.identifier.citationGowda Yogaraje CV, Senthilkumar S, Kashinath RT. Correlation of uric acid levels and purine metabolism enzyme activities in plasma and liver tissues of diabetic rats. Indian Journal of Biochemistry and Biophysics. 2019 Dec; 56(6): 439-444en_US
dc.identifier.issn0975-0959
dc.identifier.issn0301-1208
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/190075
dc.languageenen_US
dc.publisherNISCAIR-CSIR, Indiaen_US
dc.relation.issuenumber6en_US
dc.relation.volume56en_US
dc.source.urihttps://nopr.niscair.res.in/handle/123456789/52172en_US
dc.titleCorrelation of uric acid levels and purine metabolism enzyme activities in plasma and liver tissues of diabetic ratsen_US
dc.typeJournal Articleen_US
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