Cloning and expression of a cell wall associated protein gene of Mycobacterium tuberculosis.

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dc.contributor.authorMohamed, A Jen_US
dc.contributor.authorJayaraman, Gen_US
dc.date.accessioned1999-04-20en_US
dc.date.accessioned2009-05-29T13:30:48Z
dc.date.available1999-04-20en_US
dc.date.available2009-05-29T13:30:48Z
dc.date.issued1999-04-20en_US
dc.description.abstractGenomic library of Mycobacterium tuberculosis (SIHV) was constructed into T7 promoter based vector pBluescript II KS (+/-) in Escherichia coil. The expression of mycobacterial antigens from recombinants was detected by immunoscreening using E-coli-preabsorbed hyperimmune rabbit anti-M tuberculosis cell wall associated proteins serum. Among 980 clones tested by immunoscreening, a clone pJJ5 was found to produce mycobacterial antigen. This clone was further characterized by immunoblotting and found to produce a 36 kDa of cell wall associated antigen Detection of antibodies response to this antigen in the serum of the majority of the individuals with tuberculosis indicates that this antigen is capable of stimulating a humoral immune response.en_US
dc.description.affiliationDepartment of Genetics, University of Madras, Taramani.en_US
dc.identifier.citationMohamed AJ, Jayaraman G. Cloning and expression of a cell wall associated protein gene of Mycobacterium tuberculosis. Indian Journal of Pathology & Microbiology. 1999 Apr; 42(2): 135-43en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/75728
dc.language.isoengen_US
dc.source.urihttps://www.ijpmonline.orgen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntigens, Bacterial --geneticsen_US
dc.subject.meshBacterial Proteins --geneticsen_US
dc.subject.meshCell Wall --geneticsen_US
dc.subject.meshCloning, Molecularen_US
dc.subject.meshEscherichia coli --geneticsen_US
dc.subject.meshGene Expressionen_US
dc.subject.meshGenes, Bacterialen_US
dc.subject.meshMycobacterium tuberculosis --geneticsen_US
dc.subject.meshRabbitsen_US
dc.subject.meshRecombinant Proteins --geneticsen_US
dc.titleCloning and expression of a cell wall associated protein gene of Mycobacterium tuberculosis.en_US
dc.typeJournal Articleen_US
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