Morphological and functional characteristics of rabbit uterine epithelial cells grown on free floating collagen gel.

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dc.contributor.authorVenkataraman, Len_US
dc.contributor.authorUpadhyay, Sen_US
dc.contributor.authorLalitkumar, P Gen_US
dc.contributor.authorSengupta, Jen_US
dc.contributor.authorGhosh, Den_US
dc.date.accessioned2001-04-02en_US
dc.date.accessioned2009-06-01T07:04:59Z
dc.date.available2001-04-02en_US
dc.date.available2009-06-01T07:04:59Z
dc.date.issued2001-04-02en_US
dc.description.abstractIn the present study isolated uterine epithelial cells from normal rabbits were maintained in culture on free floating rat-tail collagen matrix, and the morphological characteristics of these cells were examined. Additionally, the pattern of protein synthesis and secretion by rabbit uterine epithelial cells grown on free floating collagen gels following estradiol and/or progesterone treatment in vitro was examined. Isolated epithelial cells cultured on collagen gels in complete medium containing serum attached to form monlayers, and eventually the gels became free floating and contracted giving rise to luminal arrangements. These cells were cytokeratin positive epithelial cells and were ultrastructurally polarized. These cells also exhibited differential upregulation and down regulation in the synthesis and secretion of proteins in response to estradiol, progesterone, and estradiol plus progesterone. Additionally, a permissive action between progesterone and estradiol in the synthesis of two species of secretory proteins was observed. It however remains to be examined whether different species of proteins produced in vitro in response to estradiol and progesterone bear any association with physiological states in reproductive cycle in this species.en_US
dc.description.affiliationDepartment of Physiology, All India Institute of Medical Sciences, New Delhi-110 029.en_US
dc.identifier.citationVenkataraman L, Upadhyay S, Lalitkumar PG, Sengupta J, Ghosh D. Morphological and functional characteristics of rabbit uterine epithelial cells grown on free floating collagen gel. Indian Journal of Physiology and Pharmacology. 2001 Apr; 45(2): 161-71en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/107882
dc.language.isoengen_US
dc.source.urihttps://www.ijpp.comen_US
dc.subject.meshAnimalsen_US
dc.subject.meshCell Divisionen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshCollagenen_US
dc.subject.meshElectrophoresis, Polyacrylamide Gelen_US
dc.subject.meshEpithelial Cells --cytologyen_US
dc.subject.meshFemaleen_US
dc.subject.meshGelsen_US
dc.subject.meshProtein Biosynthesisen_US
dc.subject.meshRabbitsen_US
dc.subject.meshUterus --cytologyen_US
dc.titleMorphological and functional characteristics of rabbit uterine epithelial cells grown on free floating collagen gel.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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