Emergence of non-albicans Candida among candidal vulvovaginitis cases and study of their potential virulence factors, from a tertiary care center, North India.
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Date
2013-04
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Abstract
Purpose: The purpose of this study was to determine the prevalence of various
Candida species and study some of their virulence factors among thevulvovaginal
candidiasis(VVC)patients. Study Design and Settings: The study was conducted
in a Tertiary Care University Hospital in North India. Materials and Methods:
This study was carried out prospectively for a period of 1 year. High vaginal
swabs (HVSs) were collected from women in childbearing age group attending
the gynecology and obstetrics out-patient departments with the complaints
suggestive of vulvovaginitis. Samples were plated on Sabouraud’s dextrose
agar slope. Candida spp. isolated was further speciated based on microscopy,
biochemical tests and culture characteristics on special media. Virulence factors
of these strains were determined by biofi lm formation and phospholipase activity.
Result: A total of 464 HVS from 232 patients with the complaints of vulvovaginitis
were included in this study. Following laboratory workup, 71 specimens were
positive for genus Candida (30.6%). Further speciation showed 32.4% as
Candida albicans, 45.07% Candida parapsilosis and 22.53% of Candida glabrata.
Biofi lm production was shown by 50 candidal strains (70.4%) and phospholipase
activity was given by 41 candidal strains (57.74%). Conclusion: Our study
suggests increasing prevalence of non-albicans Candida among the VVC cases
along with their virulence factors. Therefore, we recommend that microbiological
investigation upto species level should be mandatory to determine the emergence
of non-albicans Candida as a major cause of VVC.
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Keywords
Biofi lm, non-albicans, vulvovaginal candidiasis
Citation
Kumari Varsha, Banerjee Tuhina, Kumar Pankaj, Pandey Sulekha, Tilak Ragini. Emergence of non-albicans Candida among candidal vulvovaginitis cases and study of their potential virulence factors, from a tertiary care center, North India. Indian Journal of Pathology & Microbiology. 2013 Apr-Jun 56 (2): 144-147.