A simple alternative method for rapid detection of slime produced by Staphylococcus epidermidis isolates in bacterial keratitis.

Simple item page
dc.contributor.authorNayak, Nen_US
dc.contributor.authorSatpathy, Gen_US
dc.contributor.authorVajpayee, R Ben_US
dc.contributor.authorPandey, R Men_US
dc.date.accessioned2001-11-25en_US
dc.date.accessioned2009-05-27T07:25:03Z
dc.date.available2001-11-25en_US
dc.date.available2009-05-27T07:25:03Z
dc.date.issued2001-11-25en_US
dc.description.abstractBACKGROUND & OBJECTIVES: Slime is a known virulence factor of Staphylococcus epidermidis. The conventional Christensen's method for detection of slime in the laboratory takes at least 48 h. We, therefore, tried to evaluate the efficacy of the Congo red agar method as a routine procedure for detecting slime among isolates from corneal ulcers. METHODS: A total of 244 isolates from corneal ulcers were identified as S. epidermidis by the standard procedures. Slime was detected both by the conventional Christensen's method as well as by the Congo red agar method. RESULTS: Ninety two (37.7%) isolates were positive and 86 (35.2%) were negative for slime by both the techniques. Fifty four (22.1%) isolates were positive in Congo red agar, but negative by Christensen's method; whereas only 12 (4.9%) were negative by Congo red but positive by Christensen's method. Detection of slime by Congo red agar method was rapid i.e., all the 146 strains were positive within 24 h of incubation. On the other hand, Christensen's method had a delayed response; 42.3 per cent (44/104) strains being negative during the first 24 h of incubation. INTERPRETATION & CONCLUSION: Our results suggested that culture on Congo red agar was a sensitive and rapid test for detecting slime. This might help in the quick identification in a routine laboratory of slime positive isolates in bacterial keratitis.en_US
dc.description.affiliationDivision of Microbiology, Dr. Rajendra Prasad Centre for Ophthalmic Sciences, All India Institute of Medical Sciences, New Delhi, India.en_US
dc.identifier.citationNayak N, Satpathy G, Vajpayee RB, Pandey RM. A simple alternative method for rapid detection of slime produced by Staphylococcus epidermidis isolates in bacterial keratitis. Indian Journal of Medical Research. 2001 Nov; 114(): 169-72en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/20621
dc.language.isoengen_US
dc.source.urihttps://icmr.nic.in/ijmr/ijmr.htmen_US
dc.subject.meshBacteriological Techniquesen_US
dc.subject.meshCongo Reden_US
dc.subject.meshCorneal Ulcer --microbiologyen_US
dc.subject.meshHumansen_US
dc.subject.meshPolysaccharides, Bacterial --biosynthesisen_US
dc.subject.meshStaphylococcal Infections --microbiologyen_US
dc.subject.meshStaphylococcus epidermidis --isolation & purificationen_US
dc.subject.meshVirulenceen_US
dc.titleA simple alternative method for rapid detection of slime produced by Staphylococcus epidermidis isolates in bacterial keratitis.en_US
dc.typeComparative Studyen_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
Files
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.79 KB
Format:
Plain Text
Description:
Download
Collections
Indian Journal of Medical Research