In vitro and in vivo evaluation of antibiotic combination against colistin resistant Acinetobacter baumannii isolated from patients of a tertiary care hospital, Bangladesh

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dc.contributor.authorJahan, Sen_US
dc.contributor.authorShamsuzzaman, SM.en_US
dc.date.accessioned2025-05-12T07:25:27Z
dc.date.available2025-05-12T07:25:27Z
dc.date.issued2024-10
dc.description.abstractBackground: Emerging resistance to colistin in clinical Acinetobacter baumannii isolates is of growing concern as these-strain are simultaneously resistant to most antimicrobial agents. Colistin is considered as last resort for treating severe bacterial infections caused by gram negative multidrug resistant bacteria. Since current treatment option for these strains are extremely limited, different antimicrobial combinations were used to see their efficacy against colistin resistant A. baumannii both in vitro and in vivo. Methods: Cross-sectional study was done in the department of Microbiology, Dhaka Medical College, Bangladesh from July, 2017 to June, 2018. Antibiotic susceptibility was performed by disc-diffusion technique and MIC. Colistin resistance genes were detected by PCR. In vitro activity of colistin, tigecycline, imipenem, amikacin and their combinations were evaluated by agar dilution method. Rat septicemic models were made using the colistin resistant A. baumannii and effectiveness of antibiotic combinations were tested in rats. Results: Among 32 isolated A. baumannii 12.5% were colistin resistant. All colistin resistant A. baumannii were positive for lpxA, lpxC, pmrA, pmrB genes; 75% were positive for lpxD gene, 50% were positive for pmrC gene. The proportions of synergy observed in colistin-tigecycline, colistin-imipenem, colistin-amikacin and imipenem-amikacin were 75%, 50%, 50% and 25% respectively in vitro. The best in vivo result appeared in group treated with colistin-tigecycline combination where 100% bacterial clearance from rats was observed while colistin-imipenem combination showed 83.33% clearance. Conclusion: Colistin plus tigecycline or colistin plus imipenem may be alternative treatment option against colistin resistant A. baumannii infection.en_US
dc.identifier.affiliationsInstitute of Public Health, Mohakhali, Dhaka, Bangladeshen_US
dc.identifier.affiliationsDepartment of Microbiology, Dhaka Medical College, Dhaka, Bangladeshen_US
dc.identifier.citationJahan S, Shamsuzzaman SM.. In vitro and in vivo evaluation of antibiotic combination against colistin resistant Acinetobacter baumannii isolated from patients of a tertiary care hospital, Bangladesh . International Journal of Research in Medical Sciences. 2024 Oct; 12(10): 3607-3614en_US
dc.identifier.issn2320-6071
dc.identifier.issn2320-6012
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/246833
dc.languageenen_US
dc.publisherMedip Academyen_US
dc.relation.issuenumber10en_US
dc.relation.volume12en_US
dc.source.urihttps://doi.org/10.18203/2320-6012.ijrms20242916en_US
dc.subjectAcinetobacter baumanniien_US
dc.subjectcolistin resistanceen_US
dc.subjectcombined antibiotic therapyen_US
dc.subjectin vitro and in vivo efficacyen_US
dc.titleIn vitro and in vivo evaluation of antibiotic combination against colistin resistant Acinetobacter baumannii isolated from patients of a tertiary care hospital, Bangladeshen_US
dc.typeJournal Articleen_US
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