Production of polyclonal antibody against recombinant ORF 112 L of rock bream (Oplegnathus fasciatus) iridovirus (RBIV) and in vitro neutralization.

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dc.contributor.authorKim, Yun Imen_US
dc.contributor.authorHa, Yu Mien_US
dc.contributor.authorNam, Yoon Kwonen_US
dc.contributor.authorKim, Ki Hongen_US
dc.contributor.authorKim, Sung Kooen_US
dc.date.accessioned2008-07-07en_US
dc.date.accessioned2009-06-02T03:49:48Z
dc.date.available2008-07-07en_US
dc.date.available2009-06-02T03:49:48Z
dc.date.issued2008-07-07en_US
dc.description.abstractRock bream iridovirus (RBIV) is a causative agent of epizootics among cultured rock bream (Oplegnathus fasciatus) in Korea. In this study, the immunogenic property of ankyrin repeats gene (ORF 112L) from RBIV was evaluated to develop vaccines against RBIV. ORF 112L of RBIV was cloned into expression vector of pGEX-4T-1. The recombinant protein was successfully expressed using E. coli BL21 (DE3). The soluble recombinant RBIV protein was applied to affinity column for the purification of the protein. Mice were immunized by the injection of purified recombinant protein to produce polyclonal antibodies. EUSA was carried out to identify the immune reaction abilities of polyclonal antibody to recombinant protein. The antigenic property of this protein was evaluated by using in vitro neutralization with BF-2 cells. In neutralization test, BF-2 cells infected with the mixture of RBIV and antisera containing anti-GST-ORF 112L polyclonal antibody were healthy showing few cytopathic effect (CPE) similar with the negative control (without RBIV). These studies suggest that the protein from the ankyrin repeats gene, ORF 112L of RBIV may play an important role in the mechanism of infection. Also, it can be possible to develop protein or gene vaccines using ORF 112L against RBIV.en_US
dc.description.affiliationDepartment of Biotechnology and Bioengineering, Pukyong National University, Busan, 608-737, Korea.en_US
dc.identifier.citationKim YI, Ha YM, Nam YK, Kim KH, Kim SK. Production of polyclonal antibody against recombinant ORF 112 L of rock bream (Oplegnathus fasciatus) iridovirus (RBIV) and in vitro neutralization. Journal of Environmental Biology. 2008 Jul; 29(4): 571-6en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/113341
dc.language.isoengen_US
dc.source.urihttps://www.geocities.com/j_environ_biol/en_US
dc.subject.meshAmino Acid Sequenceen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntibodies, Viral --geneticsen_US
dc.subject.meshElectrophoresisen_US
dc.subject.meshEnzyme-Linked Immunosorbent Assayen_US
dc.subject.meshImmune Sera --immunologyen_US
dc.subject.meshIridovirus --geneticsen_US
dc.subject.meshMiceen_US
dc.subject.meshMice, Inbred BALB Cen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshNeutralization Testsen_US
dc.subject.meshPerciformes --virologyen_US
dc.subject.meshRecombinant Proteins --geneticsen_US
dc.subject.meshSequence Alignmenten_US
dc.subject.meshViral Vaccines --administration & dosageen_US
dc.titleProduction of polyclonal antibody against recombinant ORF 112 L of rock bream (Oplegnathus fasciatus) iridovirus (RBIV) and in vitro neutralization.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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