Construction, purification and characterization of a recombinant baculovirus containing the gene for alpha subunit of human chorionic gonadotropin.

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dc.contributor.authorNakhai, Ben_US
dc.contributor.authorSridhar, Pen_US
dc.contributor.authorTalwar, G Pen_US
dc.contributor.authorHasnain, S Een_US
dc.date.accessioned1991-08-01en_US
dc.date.accessioned2009-05-27T09:02:57Z
dc.date.available1991-08-01en_US
dc.date.available2009-05-27T09:02:57Z
dc.date.issued1991-08-01en_US
dc.description.abstractA cDNA encoding the alpha subunit of human chorionic gonadotropin, a placental glycoprotein hormone, was cloned downstream to the viral polyhedrin gene promoter of Autographa california nuclear polyhedrosis virus and the recombinant transfer vector was used to co-transfect Spodoptera frugiperda cells growing in culture. Recombinant baculovirus carrying the alpha hCG gene was detected and isolated after dot hybridization using supernatant from co-transfected cells. Recombinant vAc alpha hCG having a replacement of the viral polyhedrin gene, which is hyper-transcribed very late in the infection cycle, with the alpha hCG cDNA was purified after a single round of plaque purification. Insect cell culture infected with vAc alpha hCG, secreted high levels of hCG which was biologically active.en_US
dc.description.affiliationNational Institute of Immunology, Shahid Jeet Singh Marg, New Delhi, India.en_US
dc.identifier.citationNakhai B, Sridhar P, Talwar GP, Hasnain SE. Construction, purification and characterization of a recombinant baculovirus containing the gene for alpha subunit of human chorionic gonadotropin. Indian Journal of Biochemistry & Biophysics. 1991 Aug; 28(4): 237-42en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/26280
dc.language.isoengen_US
dc.source.urihttps://https://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijbb/ijbb0.aspen_US
dc.subject.meshBaculoviridae --geneticsen_US
dc.subject.meshCloning, Molecularen_US
dc.subject.meshDNA --geneticsen_US
dc.subject.meshGenetic Vectorsen_US
dc.subject.meshGlycoprotein Hormones, alpha Subunit --geneticsen_US
dc.subject.meshHumansen_US
dc.subject.meshRestriction Mappingen_US
dc.titleConstruction, purification and characterization of a recombinant baculovirus containing the gene for alpha subunit of human chorionic gonadotropin.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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