Action of K-crown ether on photosystem II electron transport: characterization of the site of action.

Simple item page
dc.contributor.authorMohanty, Pen_US
dc.contributor.authorSeibert, Men_US
dc.date.accessioned1997-06-01en_US
dc.date.accessioned2009-05-27T09:36:33Z
dc.date.available1997-06-01en_US
dc.date.available2009-05-27T09:36:33Z
dc.date.issued1997-06-01en_US
dc.description.abstractWe have investigated the inhibitory effect of K-crown (18-crown-6 potassium picrate) on photosystem II (PSII)-enriched membrane fragments and O2-evolving core complexes. K-crown at 2-4 microM inhibits about half the control level of O2-evolution activity in both types of PSII samples. Oxygen-evolution studies demonstrated that the ether works by inactivating the centres and not by interfering with antenna function or energy transfer to the reaction centre. K-crown does not disrupt binding of the extrinsic proteins associated with O2 evolution nor complex with bound Ca2+ or Cl- cofactors, but rather it directly inhibits electron transfer after the tetrameric Mn cluster. Fluorescence studies on active and Tris-treated samples showed that K-crown does not prevent artificial donors from transferring electrons to PSII but like DCMU inhibits on the acceptor side after QA, the primary quinone acceptor. However, the ether is a leaky inhibitor and may also act as a weak donor when the Mn cluster is not present. Oxygen-production experiments using silicomolybdate as an artificial acceptor (which accepts from both pheophytin and QB in PSII membranes) demonstrated that the inhibition is at or near the DCMU site.en_US
dc.description.affiliationSchool of Life Sciences, Jawaharlal Nehru University, New Delhi, India.en_US
dc.identifier.citationMohanty P, Seibert M. Action of K-crown ether on photosystem II electron transport: characterization of the site of action. Indian Journal of Biochemistry & Biophysics. 1997 Jun; 34(3): 241-8en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/28020
dc.language.isoengen_US
dc.source.urihttps://https://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijbb/ijbb0.aspen_US
dc.subject.meshBinding Sitesen_US
dc.subject.meshChlorophyll --metabolismen_US
dc.subject.meshElectron Transport --drug effectsen_US
dc.subject.meshEthers, Cyclic --pharmacologyen_US
dc.subject.meshEthyldimethylaminopropyl Carbodiimide --pharmacologyen_US
dc.subject.meshKineticsen_US
dc.subject.meshLighten_US
dc.subject.meshLight-Harvesting Protein Complexesen_US
dc.subject.meshMolybdenum --metabolismen_US
dc.subject.meshOxygen --metabolismen_US
dc.subject.meshPhotosynthesis --drug effectsen_US
dc.subject.meshPhotosynthetic Reaction Center Complex Proteins --chemistryen_US
dc.subject.meshPhotosystem II Protein Complexen_US
dc.subject.meshPlant Proteins --metabolismen_US
dc.subject.meshSilicon Compounds --metabolismen_US
dc.subject.meshSpinacia oleracea --metabolismen_US
dc.titleAction of K-crown ether on photosystem II electron transport: characterization of the site of action.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, U.S. Gov't, Non-P.H.S.en_US
Files
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.79 KB
Format:
Plain Text
Description:
Download
Collections
Indian Journal of Biochemistry & Biophysics