Antipathogenic potential of marine Bacillus sp. SS4 on N-acyl-homoserine-lactone-mediated virulence factors production in Pseudomonas aeruginosa (PAO1).
Loading...
Date
2011-03
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Antipathogenic therapy is an outcome of the quorum-sensing inhibition (QSI) mechanism, which targets autoinducerdependent
virulent gene expression in bacterial pathogens. N-acyl homoserine lactone (AHL) acts as a key regulator
in the production of virulence factors and biofilm formation in Pseudomonas aeruginosa PAO1 and violacein
pigment production in Chromobacterium violaceum. In the present study, the marine bacterial strain SS4 showed
potential QSI activity in a concentration-dependent manner (0.5–2 mg/ml) against the AHL-mediated violacein
production in C. violaceum (33–86%) and biofilm formation (33–88%), total protease (20–65%), LasA protease (59–
68%), LasB elastase (36–68%), pyocyanin (17–86%) and pyoverdin productions in PAO1. The light and confocal laser
scanning microscopic analyses confirmed the reduction of the biofilm-forming ability of PAO1 when treated with SS4
extract. Furthermore, the antibiofilm potential was confirmed through static biofilm ring assay, in which ethyl acetate
extract of SS4 showed concentration-dependent reduction in the biofilm-forming ability of PAO1. Thus, the result of
this study clearly reveals the antipathogenic and antibiofilm properties of the bacterial isolate SS4. Through 16S rDNA
analysis, the strain SS4 was identified as Bacillus sp. (GenBank Accession Number: GU471751).
Description
Keywords
Antipathogenic therapy, Bacillus sp, Chromobacterium violaceum, N-acyl homoserine lactone, Pseudomonas aeruginosa, virulence genes
Citation
Musthafa K Syed, Saroja V, Pandian S Karutha, Ravi A Veera. Antipathogenic potential of marine Bacillus sp. SS4 on N-acyl-homoserine-lactone-mediated virulence factors production in Pseudomonas aeruginosa (PAO1). Journal of Biosciences. 2011 Mar; 36(1): 55-67.