Evaluation of direct agglutination test (DAT) as an immunodiagnostic tool for diagnosis of visceral leishmaniasis in Nepal.

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dc.contributor.authorJoshi, A Ben_US
dc.contributor.authorSinghasivanon, Pen_US
dc.contributor.authorKhusmith, Sen_US
dc.contributor.authorFungladda, Wen_US
dc.contributor.authorNandy, Aen_US
dc.date.accessioned2009-05-27T15:14:58Z
dc.date.available2009-05-27T15:14:58Z
dc.date.issued1999-09-25en_US
dc.descriptionThe Southeast Asian Journal of Tropical Medicine and Public Health.en_US
dc.description.abstractBefore field application of the direct agglutination test (DAT) for leishmaniasis, it was assessed as a diagnostic tool. Fifteen confirmed visceral leishmaniasis cases (bone marrow aspiration positive), 120 tuberculosis, 58 leprosy, 15 malaria, 26 intestinal parasitic infection cases, 24 endemic healthy controls from adjacent to the study area, and 18 controls from Kathmandu (who had never visited the VL endemic areas) were tested for anti-leishmanial antibody agglutination titers. Two of the tuberculosis cases were positive for anti-leishmanial agglutinating antibodies at 1:800. All the visceral leishmaniasis confirmed cases were reactive to anti-leishmanial antibody at > or = 1:3,200. Other specimens were negative for serology. The sensitivity of the direct agglutination test was 100% and the specificity was 99.2%. The direct agglutination test had positive and negative predictive values of 100% and 99.2% respectively. The direct agglutination test has been found to be simple, rapid, reliable, economic, safe and adaptable to micro-techniques using microtiter plates. It is specific and sensitive. The direct agglutination test is simple enough for it to be performed in a field laboratory.en_US
dc.description.affiliationDepartment of Community Medicine and Family Health, Institute of Medicine, Tribhuvan University, Nepal.en_US
dc.identifier.citationJoshi AB, Singhasivanon P, Khusmith S, Fungladda W, Nandy A. Evaluation of direct agglutination test (DAT) as an immunodiagnostic tool for diagnosis of visceral leishmaniasis in Nepal. The Southeast Asian Journal of Tropical Medicine and Public Health. 1999 Sep; 30(3): 583-5en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/32300
dc.language.isoengen_US
dc.source.urihttps://www.tm.mahidol.ac.th/seameo/1999-30-3/1999-30-3-583.pdfen_US
dc.subject.meshAgglutination Tests --methodsen_US
dc.subject.meshEvaluation Studies as Topicen_US
dc.subject.meshHumansen_US
dc.subject.meshLeishmaniasis, Visceral --diagnosisen_US
dc.subject.meshNepal --epidemiologyen_US
dc.subject.meshPredictive Value of Testsen_US
dc.subject.meshSensitivity and Specificityen_US
dc.titleEvaluation of direct agglutination test (DAT) as an immunodiagnostic tool for diagnosis of visceral leishmaniasis in Nepal.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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