To Assess the Correlation between Bone Marrow Morphology and Immunophenotypic Findings in Patients of Chronic Lymphoproliferative Disorders (CLPD’s) and to Assess the Role of Flowcytometric Immunophenotyping in Diagnosis and Subclassification of CLPD’s

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dc.contributor.authorDwivedi, Ektaen_US
dc.contributor.authorKushwaha, Rashmien_US
dc.contributor.authorJain, Milien_US
dc.contributor.authorYadav, Geetaen_US
dc.contributor.authorVerma, S.P.en_US
dc.contributor.authorSingh, U.Sen_US
dc.contributor.authorKumar, Ashutoshen_US
dc.date.accessioned2020-09-24T07:10:24Z
dc.date.available2020-09-24T07:10:24Z
dc.date.issued2019-07
dc.description.abstractIntroduction: Chronic lymphoproliferative disorderrepresent clonal proliferation of morphologically andimmunophenotypically mature B or T cells characterized by alow proliferation rate and prolonged cell survival. Study aimedto assess the correlation between bone marrow morphologyand immunophenotypic findings in patients of ChronicLymphoproliferative Disorders (CLPD’s) and to assess therole of flowcytometric immunophenotyping in diagnosis andsubclassification of CLPD’s.Material and Methods: 48 newly diagnosed cases ofCLPD were included. After complete clinical evaluation theyunderwent marrow aspiration, biopsy and immunophenotypingby flowcytometry with selected panel of monoclonalantibodies.Results: On morphology 47.9% cases were CLL. In 52.1%non CLL cases , 4.2% were PLL , 2% case as LPL and45.8% cases were CLPD-unclassifiable. Commonest patternof marrow infiltration noted on trephine biopsy was diffuse inCLL, HCL-V, B-PLL and T-CLPD. On immunophenotyping95.8% cases were B-CLPD and 4.25% T-CLPD. CD5, CD22,CD23, FMC7 and SmIg were used as first line markersfollowed by CD 10, CD 25, CD103, CD38, CD138 andCyclin D1 (on biopsy sections) as second line markers. Finalimmunophenotypic diagnosis was CLL (54.2%), B-CLPDunclassified (29.2%), 4.1% each of LPL, MCL, T-CLPD and2% each of B-PLLand HCL-V.Conclusion: Concordance rate between morphologicaldiagnosis and immunophenotypic diagnosis was 79.17%.Hence, Flowcytometry is necessary for confirmationof diagnosis and to classify the CLPD cases which areunclassifiable by morphologyen_US
dc.identifier.affiliationsJR, Department of Pathology, K.G.M.U, Lucknowen_US
dc.identifier.affiliationsProfessor, Department of Pathology, K.G.M.U, Lucknowen_US
dc.identifier.affiliationsAssistant Professor, Department of Pathology, K.G.M.U, Lucknowen_US
dc.identifier.affiliationsAssistant Professor, Department of Pathology, K.G.M.U, Lucknowen_US
dc.identifier.affiliationsAssociate Professor, Department of Clinical Hematology, K.G.M.U, Lucknowen_US
dc.identifier.affiliationsProfessor, Department of Pathology, K.G.M.U, Lucknowen_US
dc.identifier.affiliationsHead and Professor, Department of Pathology, K.G.M.U, Lucknow, Indiaen_US
dc.identifier.citationDwivedi Ekta, Kushwaha Rashmi, Jain Mili, Yadav Geeta, Verma S.P., Singh U.S, Kumar Ashutosh. To Assess the Correlation between Bone Marrow Morphology and Immunophenotypic Findings in Patients of Chronic Lymphoproliferative Disorders (CLPD’s) and to Assess the Role of Flowcytometric Immunophenotyping in Diagnosis and Subclassification of CLPD’s. International Journal of Contemporary Medical Research . 2019 Jul; 6(7): g5-g11en_US
dc.identifier.issn2393-915X
dc.identifier.issn2454-7379
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/202453
dc.languageenen_US
dc.publisherInternational Society for Contemporary Medical Researchen_US
dc.relation.issuenumber7en_US
dc.relation.volume6en_US
dc.source.urihttps://dx.doi.org/10.21276/ijcmr.2019.6.7.19en_US
dc.subjectCLPDen_US
dc.subjectMorphology; CLPDen_US
dc.subjectImmunophenotype; CLPDen_US
dc.subjectFlowcytometryen_US
dc.titleTo Assess the Correlation between Bone Marrow Morphology and Immunophenotypic Findings in Patients of Chronic Lymphoproliferative Disorders (CLPD’s) and to Assess the Role of Flowcytometric Immunophenotyping in Diagnosis and Subclassification of CLPD’sen_US
dc.typeJournal Articleen_US
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